摘要
比较日粮中单独添加或同时添加黄芪多糖、葡萄籽提取物对衰老模型小鼠负重游泳时间和血常规参数的影响。采用在日粮中添加0.5%半乳糖构建衰老小鼠动物模型,并将衰老模型小鼠分为模型对照组、黄芪多糖处理组、葡萄籽提取物处理组、黄芪多糖+葡萄籽提取物处理组,每组10只。黄芪多糖和葡萄籽提取物的添加量均为0.1%。试验第42 d时进行负重游泳时间测定,第43 d时眼眶采血,检测血常规参数。结果显示与衰老模型组相比,同时添加黄芪多糖和葡萄籽提取物,可显著提高小鼠的负重游泳时间,显著降低血液粒细胞数和红细胞平均血红蛋白含量;分别单独添加黄芪多糖和葡萄籽提取物均可显著降低红细胞体积分布宽度、血红蛋白浓度和红细胞平均血红蛋白含量。说明黄芪多糖和葡萄籽提取物同时添加对衰老模型小鼠的负重游泳时间有较好延长作用,单独或同时添加黄芪多糖和葡萄籽提取物对于衰老模型小鼠部分血常规参数有一定影响。
The effects of dietary supplementation with Astragalus polysaccharides（APS） and grape seed extract（GSE）individually and together on loaded swimming time and blood parameters in aging mice model were investigated in this study. The aging mice model was induced by dietary supplementation with 0. 5% D-galactose. Forty aging mice model were randomly assigned to four treatment groups as aging model control,APS group,GSE group and APS ＋ GSE group with 10 mice in each treatment. APS and GSE both supplemented at the dose of 0. 1% of diet. Loaded swimming time and blood parameters were determined on the 42 day and 43 day respectively after the beginning of the experiment. The results showed that the mice had markedly increased loaded swimming time,decreased significantly granulocyte counts and mean corpuscular hemoglobin（MCH） in APS ＋ GSE treatment as compared with aging model control; the mice treated by APS or GSE had significantly decreased red blood cell volume distribution width（RDW）,hemoglobin（HGB）and mean corpuscular hemoglobin（MCH）. These results suggested that dietary supplementation with APS and GSE can significantly prolong loaded swimming time in aging mice model while individual or simultaneous dietary supplementation with APS and GSE can affect some blood parameters.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第1期158-162,共5页
Natural Product Research and Development
基金
安徽省教育厅自然科学研究项目(KJ2011B215)
皖西学院大学生创新创业训练计划项目(201210376036)
关键词
黄芪多糖
葡萄籽提取物
衰老
小鼠
血常规参数
Astragalus polysaccharides
grape seed extract
aging
mice
blood parameters
