摘要
通过响应面试验设计,获得超声提取黑果腺肋花楸叶多糖的最佳工艺条件,通过TCA法将粗多糖中的蛋白成分除去后得到精制多糖;以清除铁还原力、1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力和清除羟自由基能力为指标,评价黑果腺肋花楸叶多糖的抗氧化活性。结果表明,超声提取黑果腺肋花楸叶多糖的最佳工艺条件为:超声温度67℃,超声时间53 min,超声功率150W,料液比1∶30(g∶mL)在此条件下多糖得率为5.01%。黑果腺肋花楸叶多糖具有较好的抗氧化活性,铁还原力、清除DPPH自由基能力和清除羟自由基能力均表现出一定的质量浓度依赖性;黑果腺肋花楸叶多糖多糖铁还原力、清除DPPH自由基和清除羟自由基能力的半数有效质量浓度(EC50)分别为0.623g/L、0.473g/L和0.147g/L。
The conditions for ultrasonic assisted extraction of polysaccharides from Aronia melanocarpa Berries leaves were optimized by response surface methodology. The antioxidant activities of polysaccharides refined by TCA method were investigated using three indices which were iron-reducing power, 1, 1 diphenyl-2-picrylhydrazyl （DPPH） radical scavenging activity and hydroxyl radical scavenging activity . The results showed that the optimal extraction conditions were ： ultrasonic power 150W, ultrasonic time 53min, solid-to-solvent ratio 1 ： 30 and extraction temperature 67℃. The experimentally observed yield of polysaccharides extracted from Aronia melanocarpa Berries leaves was 5.01% under these conditions. The extracted polysaccharides had significant antioxidant activity, and iron-reducing power, 1, 1-diphenyl-2 picrylhydrazyl （DPPH） radical scavenging activity and hydroxyl radical scavenging activity all showed concentration-dependent manner, with median effective concentration （EC50） of 0.623, 0.473 and 0.147 g/L, respectively.
作者
周雪艳
ZHOU Xue-yan(Shijiazhuang Inspection Center for Food and Drug, Shijiazhuang 050011)
出处
《中国食品添加剂》
CAS
2018年第5期96-104,共9页
China Food Additives
关键词
黑果腺肋花楸叶
多糖
响应面
抗氧化
aronia melanocarpa berries leaves
polysaccharides
response surface methodology
antioxidant activity
