摘要
目的:探讨不同时间持续缺氧孵育后脂肪细胞脂联素和瘦素表达的变化。方法:将分化成熟的3T3-L1脂肪细胞随机分为6组,即常氧(21%O2)组和缺氧(1%O2)4、12、24、48、72 h组,实时定量PCR(q RT-PCR)法测定各时间点脂肪细胞中缺氧诱导因子1α(HIF-1α)、葡萄糖转运子1(GLUT-1)、脂联素和瘦素基因表达。结果:与常氧组相比,缺氧12、24、48 h组HIF-1α、GLUT-1 m RNA表达水平升高,差异有统计学意义(P<0.05)。缺氧24 h时HIF-1α、GLUT-1 m RNA达到最高。各缺氧组脂联素m RNA在12、24、48、72 h缺氧组的表达下降,较常氧组有统计学差异(P<0.05)。各缺氧组瘦素m RNA的表达高于常氧组,并在24 h时达到最高,差异有统计学意义(P<0.05)。结论:缺氧使脂肪细胞HIF-1α、GLUT-1、瘦素m RNA表达升高,脂联素m RNA表达下降,各反应在缺氧24 h时达到最大水平。
Objective： To explore the effect of different incubation time of hypoxia on the expression of adipokines in the 3T3-L1 adipocytes. Methods： The differentiated 3T3-L1 adipocytes were randomly divided into 6 groups, normoxia （21%O2）and hypoxia （1%O2） conditions for 4, 12, 24, 48, 72 h respectively. The mRNA expressions of hypoxia inducible factor-1α （HIF-1α）, glucose transporter 1 （GLUT-1）, adiponectin and leptin were detected by the qRT-PCR. Results： In 3T3-L1 adipocytes, the mRNA expressions of HIF-1α and GLUT-1 in hypoxia conditions for 12 h, 24 h and 48 h were higher than normoxia-treated cells （P〈0.05）; furthermore, they were highest in the group of hypoxia-treated cells for 24 h. Compared with the normoxia group, the adiponectin mRNA expressions of the hypoxia-treated 3T3-L1 adipoeytes decreased in all groups （P〈0.05） except for the 4 h group （P〈0.05）. Ttranscription level of leptin in the hypoxia-treated cells increased（P〈0.05）, and it reached the highest level in the 24 h hypoxia group. Conclusion： The mRNA expressions of HIF-1α, GLUT- 1 and leptin of 3T3-L1 adipocytes increase in the condition of hypoxia incubation, while the level of adiponectin mRNA decreases under hypoxia treatment. All changes above reach the peaks within the 24 h of hypoxia-treated 3T3-L1 adipocytes.
出处
《天津医科大学学报》
2015年第1期1-5,共5页
Journal of Tianjin Medical University
基金
国家自然科学基金资助项目(81170740
81270144)
天津市科委科技支撑项目(09ZCZDSF04500)
