摘要
目的研究解郁方抗抑郁作用的机理。方法采用酶联免疫检测试剂盒测定小鼠脑组织中单胺类神经递质含量;分光光度计法检测小鼠脑组织中单胺氧化酶(monoamine oxidase,MAO)活性,来研究解郁方抗抑郁作用的机制。结果解郁方Ⅰ和Ⅱ(12.00、6.00 g·kg-1)组均可显著升高小鼠脑内去甲肾上腺素、5-羟色胺、多巴胺(分别为P<0.01,P<0.05),解郁方Ⅰ和Ⅱ组可显著降低MAO活性(分别为P<0.01,P<0.05)。结论解郁方可能是通过抑制MAO活性,使突触间隙的单胺类神经递质含量升高,而起到抗抑郁作用。
Objective To study the mechanism of antidepressant effect of Jieyufang. Methods To study the possible mechanism,the following methods had been carried out: the content of monoamine neurotransmitter was determined by enzyme-linked immunosorbent assay(ELISA); The content of monoamine oxidase in the mouse brain was determined by spectrophotometry. Results In the Jieyufang groupⅠ,Ⅱthe content of 5-HT,DA and NE in the brain increased significantly(P < 0. 01,P < 0. 05); The content of MAO in the brain decreased significantly(P < 0. 01,P < 0. 05). Conclusion The mechanism of antidepressant may be associated with decrease the activity of MAO,increase the concentration of 5-HT,NE,DA in the brain.
出处
《药学研究》
CAS
2015年第1期4-6,共3页
Journal of Pharmaceutical Research
基金
宁夏自然科学基金项目(No.NZ0654)
关键词
抑郁症
解郁方
利血平模型
单胺类神经递质
单胺氧化酶
作用机理
Depression
Jieyufang
Reserpine model mouse
Monoamine neurotransmitter
Monoamine oxidase
Mechanism of action
