摘要
目的：建立测定红卫蛇药片中薯蓣皂苷元、黄独乙素、鬼臼毒素和山柰素含量的方法。方法：采用高效液相色谱法。色谱柱为Hypersil C18,流速为1.0 ml/min;薯蓣皂苷元和黄独乙素的流动相为乙腈-水-磷酸（67∶33∶0.5,V/V/V）,检测波长为305 nm;鬼臼毒素和山柰素的流动相为甲醇-乙腈-0.1 mol/L磷酸二氢钾水溶液（55∶40∶5,V/V/V）,检测波长为360 nm。结果：薯蓣皂苷元和黄独乙素的进样量分别在0.109 4~2.188 0、0.328 6~6.572 0μg范围内与各自峰面积积分值呈良好线性关系（r=0.999 5、0.999 2）;精密度、稳定性、重复性试验的RSD均〈1%;平均加样回收率分别为98.01%、97.14%,RSD分别为1.01%、0.79%（n=6）。鬼臼毒素和山柰素的进样量分别在0.159 8~3.196 0、0.033 4~0.668 0μg范围内与各自峰面积积分值呈良好线性关系（r=0.999 3、0.999 7）;精密度、稳定性、重复性试验的RSD均〈1%;平均加样回收率分别为98.04%、96.87%,RSD分别为1.59%、1.19%（n=6）。结论：该方法操作简便、灵敏度高、重复性好,结果准确、可靠,可用于红卫蛇药片的质量控制。
OBJECTIVE： To develop a method for the content determination of diosgenin, diosbulbin B, podophyllotoxin and kaempferol in HWSYP. METHODS： HPLC method was adopted. The determination was performed on Hypersil C18 column at the flow rate of 1.0 ml/min. The mobile phase of diosgenin and diosbulbin B consisted of acetonitrile-water-phosphoric acid （67 ： 33 ： 0.5, V/V/V）, and detection wavelength was set at 305 nm. That of podophyllotoxin and kaempferol consisted of methanol-acetonitrile-0.1 mol/L KI-IePO4（55 ： 40： 5, V/V/V） , and detection wavelength was set at 360 nm. RESULTS： The linear range of diosgenin and diosbulbin B were 0.109 4-2.188 0 μg（r=0.999 5）and 0.328 6-6.572 0 μg（r=0.999 2）; RSDs of precision, stability and reproducibility tests were all lower than 1%; average recoveries were 98.01% （RSD=1.01% ,n=6） and 97.14% （RSD=0.79% ,n=6）. The linear range of podophyllotoxin and kaempferol were 0,159 8-3.196 0 μg（r=0.999 3）and 0.033 4-0.668 0 μg（r=0.999 7）; RSDs of precision, stability and reproducibility tests were all lower than 1%; average recoveries were 98.04% （RSD= 1.59%, n=6） and 96.87% （RSD=1.19%, n=6）. CONCLUSIONS： The method is simple, sensitive, accurate, reliable and reproducible, and can be used for the quality control of HWSYP.
出处
《中国药房》
CAS
北大核心
2015年第3期407-410,共4页
China Pharmacy
