摘要
目的分析胰岛素抵抗（IR）肥胖大鼠体内增食欲素A（orexinA）与脂肪组织特异性丝氨酸蛋白酶抑制剂（vaspin）的相关性，阐明IR病理过程中orexin参与脂肪代谢的可能作用机制。方法4周龄sD雄性大鼠75只，随机分为正常饮食（NC）组（n=20）和高脂肪膳食（HF）组（n=55），建立IR肥胖大鼠模型，钳夹技术评定IR及脂代谢相关指标。大鼠皮下注射orexinA（1×10^-8,1×10^-6mol／L），用酶联免疫吸附法检测血清orexinA和vaspin。结果（1）喂养6周后，与NC组比较，HF组大鼠血糖、胰岛素、TC、TG、LDL-C均增高（P均〈0．05）；与NC组比较，GIR60～120降低[（16.31±1．54）VS（30．22±2．76）mg／（kg·min），P〈0．05]；（2）HF组较NC组血清vaspin水平增加177．08％（P〈0．05）；皮下注射orexinA（1×10^-8，1×l0^-7，1×10^-6mol／L），NC组大鼠血清vaspin分别增加25．00％、68．75％、120，83％，HF组大鼠血清vaspin分别增加7．52％、24．06％、40．60％；（3）IR肥胖大鼠vaspin与orexinA、血糖、胰岛素、TC、TG、LDL—C呈正相关（r分别为0．482、0．515、0．303、0．388、0．255、0．253，P=0．04），与HDL—C呈负相关（r=-0．226，P均〈0．05）。结论高脂膳食诱导的IR肥胖大鼠机体内orexinA与vaspin密切相关，orexinA增强vaspin的表达，进而参与肥胖大鼠机体内IR的发生。
Objective To investigate the correlation between orexin A and the serine proteinase inhibitor vaspin in obese rats with insulin resis- tance （IR） induced by high-fat diet and elaborate the possible action mechanism of orexin involvement in fat metabohsm in IR pathological process. Methods A total of 75 4-week-old male Sprague-Dawley rats were randomly divided into the normal dietary group （NC group, n =20） and the high -fat dietary group （ HF group, n =55 ） to establish the model of obese rats with IR, the euglycemic insulin clamp technique was used to determine re- lated indicators of insulin resistance and lipid metabolism. The rats were treated with orexin A （ 1 × 10^-8 - 1 × 10^-6mol/L） by hypodermic injection. The serum levels of orexin A and vaspin in rats were detected with enzyme linked immunosorbent assay. Results After 6 weeks of high-fat diet, the se- rum glucose, insulin, TC, TG and LDL-C were increased significantly in HF group than in NC group, while GIR60-120 in obese rats was decreased significantly [ （16.31±1.54） vs （30.22±2.76）mg/（kg. min）, P 〈 0.05 ]. The serum vaspin level was increased 177.08% in HF group compared with NC group （P 〈 0.05 ）. With hypodermic injection of orexin A （ 1 × 10^-8 mol/L, 1 × 10^-7 mol/L and 1 × 10^-6 mol/L）, the levels of serum vaspin in- creased 25.00%, 68.75%, and 120.83% in NC group and increased 7.52%, 24.06%, and 40.60% in HF group. There was a positive correlation between vaspin and orexin A, glucose, insulin, TC, TG, and LDL-C （r1=0A82, P1 = 0.02, r2=0.515, P2 = 0.02, r3 =0.303, P3 = 0.04, r4=0.388,r4 = 0.03, r5=0.255, P5 = 0.04, r6=0.253, P6 = 0.04） and a negative correlation between vaspin and HDL-C （r =-0.226, P = 0.04） in obese rats with IR. Conclusion High-fat diet can induce insulin resistance and obesity in rats, and orexin A is closely correlated to vaspin in obese rats with insu- lin resistance. Orexin A increases serum vaspin expression and thus involves in onset of insulin resistance in obese ra
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2015年第2期105-108,共4页
Journal of China Medical University
基金
国家自然科学基金(81470998
81071460
81271996)
