摘要
为制备猪瘟特异性抗体,将猪瘟兔化弱毒E2基因序列修饰后克隆到pFastBac1质粒载体,经转座、转染后构建重组杆状病毒,并用纯化的重组猪瘟E2蛋白免疫大耳白兔制备特异性抗体。实验结果表明成功获得能分泌重组猪瘟E2蛋白的杆状病毒,使用纯化的重组猪瘟E2蛋白制备的猪瘟抗体具有良好的特异性和敏感性,为猪瘟病毒荧光抗体染色液的制备奠定了基础。
In order to prepare antibody for detection of classical swine fever virus（ CSFV） , the E2 gene of vaccine strain hog cholera lapinised virus （ HCLV ） was cloned into vector pFastBac1, through thransposon and transfection, a recombinant baculovirus carried HCLV E2 gene was constructed and recombinant E2 protein was expressed. After immuning rabbits with recombinant E2 protein expressed by baculovirus, the antibodies against CSFV was prepared. The results showed that the recombinant baculovirus could secret E2 protein, and the antibodies had good specificity and could be used to prepare detection reagent for CSFV.
出处
《中国兽药杂志》
北大核心
2015年第1期6-9,共4页
Chinese Journal of Veterinary Drug
基金
农业部"948"项目(2006-G57)
