摘要
从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1,sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。
A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by 60 Co γ-ray. Compared to its wild type,the mutant has not only split glumes,but also characterized by early heading,lower plant height,reduced seed setting rate and degenerated spikelets,which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene,temporally designated as split glume 1 （sg1 ）,and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1 ,where six ORFs in total were predicted.On the basis of the location of SG1 ,it is deduced that SG1 is a novel gene regulating the rice glume development.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2015年第1期22-27,共6页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31171158)
农业部转基因专项(2011ZX08009-003-005)
江苏省自然科学基金资助项目(BK2012684
BK20141291)
江苏省六大人才高峰项目
江苏高校优势学科建设工程项目
江苏省农业科技自主创新资金资助项目[CX(13)5073]
