摘要
为实现呋喃唑酮代谢物3-氨基-2-恶唑烷酮（3-amino-2-oxazolidinone,AOZ）的快速定量检测,制备AOZ-牛血清蛋白单克隆抗体,建立2种分别基于抗3-（4-羧基苯亚甲基）-氨基-2-恶唑烷酮（CPAOZ）、抗AOZ的单克隆抗体酶联免疫检测试剂盒.CPAOZ检测试剂盒在1.0～100.0 ng/mL范围具有较好的线性,IC50值为14.6 ng/mL,检测限为6.56 ng/mL,回收率为97.6％～101.3％;AOZ检测试剂盒在0.5～12.5 ng/mL范围具有良好线性,IC50值为3.9 ng/mL,检测限为0A5 ng/mL,回收率为94.1％～97A％.这2种检测试剂盒对于其他硝基呋喃类抗生素及其代谢物均不存在交叉反应.从经济、方便、快速、灵敏等因素来考虑,后者更具有发展前景.
Based on the haptens of the furazolidone metabolite 3-amino-2-oxazolidinone （AOZ） and its derivative CPAOZ,mAb-based ELISA kits for the detection of AOZ were established after the preparation of monoclonal antibodies （mAbs） against AOZ-BSA.The CPAOZ-based ELISA kit indicated excellent linearity over the range of 1.0-100.0 ng/mL,The IC50 was 14.6 ng/mL,with detection limit of 6.56 ng/mL,and the recovery for CPAOZ was 97.6％-101.3％.The AOZ-based ELISA kit exhibited excellent linearty over the range of 0.5-12.5 ng/mL,The IC50 was 3.9 ng/mL,with the detection limit of 0.45 ng/mL,and the recovery for AOZ was 94.1％-97.4％.Neither of the ELISA kits showed cross reactivity with other nitrofurans or their metabolites.Obviously,the AOZ-based ELISA kit has more promising prospects due to its advantages of economy,convenience,rapidity and sensitivity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第8期174-180,共7页
Food Science
基金
国家海洋公益性行业科研专项(201205022-3)
福建省科技重大专项(2013NZ0003)
福建省海洋与渔业厅重点项目(闽海渔合同[2010]2-27号)
关键词
3-氨基-2-恶唑烷酮
半抗原
单克隆抗体
酶联免疫检测
3-amino-2-oxazolidinone （AOZ）
hapten
monoclonal antibody （mAb）
enzyme-linked immunosorbent assay （ELISA）
