摘要
以采自山西省沁水县历山等8处植物多样性丰富的山区10个华北耧斗菜材料和5个耧斗菜园艺品种为试材,采用SRAP（sequence-related amplified polymorphism）分子标记技术进行了亲缘关系分析。结果表明：从88对引物中筛选出17对多态性丰富的引物组合用于PCR扩增,得到扩增条带352条,其中多态性条带307条,多态性比率为87.2%。利用NTSYSpc-2.0软件进行UPGMA聚类分析,分别得出15个供试材料与10个华北耧斗菜材料间的亲缘关系树状图。15个供试材料的相似系数为0.6416-0.8571,聚类结果遵循植物学分类。10个华北耧斗菜材料的相似系数为0.7218-0.8571,生境是影响华北耧斗菜聚类结果的主要因素,地理分布为次要因素。
The genetic relationship among 10 wild samples of Aquilegia yabeana from 8 main mountains in Shanxi province and 5 horticulture cuhivars of Aquilegia caerulea were analyzed with the method of SRAP Marker. The results showed that 17 pair primes among 88 were selected for PCR,and 352 clear amplified bands were obtained. The total number of polymorphism bands was amounted to 307, the polymorphism rate of genetic was 87. 2%. The 15 samples＇ matrix of genetic similarity coefficient was calculated by the software of NTSYSpc-2. 0, and the clustering dendrograrns were obtained for 15 samples and 10 samples of Aquilegia yabeana, The similarity coefficient of 15 samples was between 0. 6416-0. 8571, the samples had more relationship with botanical classification. The similarity coefficient of the 10 samples of Aquilegia yabeana was between 0. 7218 - 0. 8571, and the clustering result of these samples has more relationship with the environment and geographical distribution.
出处
《北方园艺》
CAS
北大核心
2015年第2期98-101,共4页
Northern Horticulture
基金
山西省重大科技攻关资助项目(20080311010-1)
山西农业大学青年基金资助项目(201002)
