摘要
【目的】观察电针背三针(双侧大杼、风门、肺俞)对哮喘大鼠气道重塑模型转化生长因子β1(TGF-β1)和Smad3蛋白及m RNA表达的影响,探讨电针背三针治疗哮喘的作用和分子机制。【方法】采用卵蛋白雾化法复制哮喘模型,在激发哮喘6周后处死,采用图像分析法测量大鼠气道形态学参数,免疫组织化学染色法检测支气管肺组织TGF-β1、Smad3的蛋白表达,采用实时荧光定量PCR(q RT-PCR)检测TGF-β1、Smad3 m RNA表达水平。【结果】与空白组比较,模型组大鼠平滑肌横断面的标化后面积(WAm/Pbm)、支气管内管壁横断面标化后面积(WAi/Pbm),支气管肺组织TGF-β1、Smad3蛋白及m RNA表达水平均显著升高;与模型组比较,电针组各指标均显著降低,差异均有统计学意义(P<0.05)。【结论】电针背三针对气道重塑的作用可能与其对TGF-β1/Smad3信号通路的调控有关。
Objective To observe the effect of electroacupuncture on back three-acupoints （ namely bilateral Dazhu, Fengmen, Feishu） on the protein and mRNA expression of transforming growth factor-beta 1 （TGF-β1） and Smad3 in asthmatic rat airway remodeling model, and to explore its therapeutic efficacy and molecular mechanism. Methods Rat asthma model was established by inhalation of ovalbumin. After sensitization for 6 weeks, rats were killed. And then the airway morphological parameters of rats were measured by image analysis. The protein and mRNA expression of TGF-β1 and Smad3 in lung tissues were detected by immunohistochemistry and real-time quantitative polymerase chain reaction （qRT-PCR） respectively. Results Compared with the blank group, the standardized values of muscle cross-sectional area including airway smooth muscle area （WAm） /perimeter of the basement membrane （Pbm）, and bronchial inner wall area （WAi）/Pbm were increased in the model group. The protein and mRNA expression levels of TGF-β1 and Smad3 were also increased in the model group. In electroacupuncture group, the above observation indexes were decreased, and the differences were statistically significant （ P〈0.05 compared with the model group）. Conclusion The experimental results indicated that back three-acupoint electroacupuncture has an effect on remodeling airway, and one of the mechanisms is probably associated with the regulation of TGF-β1/Smad3 signaling pathway.
出处
《广州中医药大学学报》
CAS
2015年第1期71-75,185,186,共7页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金项目(编号:81173349)
国家中医药管理局靳瑞名医工作室传承项目(编号:201059)
关键词
气道重塑
哮喘/针灸疗法
肺/病理学
基因表达调控
蛋白表达
疾病模型
动物
大鼠
Airway remodeling Asthma /acup-moxibustion therapy Lung/pathology Gene expression regulation Protein expression Disease models,animal Rats
