摘要
为将黑芥（Brassica nigra）抗病基因引入甘蓝型油菜（B.napus）,通过有性杂交合成甘蓝型油菜与黑芥的三倍体杂种,经基因组加倍获得相应的六倍体,并观察三倍体和六倍体花粉母细胞减数分裂行为。结果表明：三倍体表现为花粉完全不育,而六倍体雌配子育性得到较好恢复,雄配子育性较低（10%-20%）。三倍体减数分裂终变期染色体主要以单价体形式存在,每个细胞平均形成3.8-5.9个二价体,也有极少数三价体出现。六倍体减数分裂染色体主要形成二价体,并以较低频率形成单价体和四价体。六倍体减数分裂后期I均等分离的细胞仅占65%-85%。落后染色体、非四分孢子等异常减数分裂行为也有发生。
To introduce resistance gene of B.nigra to B.napus,Triploids between B.napus and B.nigra were synthesized via sexual cross and hexaploids were obtained by genome doubling to transfer resistance genes fromB.nigrato B.napus.Meiosis of triploids and hexaploids was also investigated by microscope.Results：Triploids were sterility at al.Majority of female gametes were fertile and minority（10%-20%）of male gametes were fertile in hexaploids.Majority of chromosomes were univalents and3.8-5.9bivalents were formed and a few of trivalents were observed in diakinesis pollen mother cells（PMCs）of triploids.Majority of chromosomes formed bivalents and univalents and tetravalents were formed in very low frequency in hexaploids.Equal segregation happened in 65%-85% anaphase I PMCs.Laggard chromosomes and non-tetragametes were observed.
出处
《贵州农业科学》
CAS
北大核心
2014年第12期27-31,共5页
Guizhou Agricultural Sciences
基金
江西省自然科学基金项目"油菜远缘杂种基因组稳定性研究"(20132BAB204015)
江西省教育厅项目"油菜种间杂种染色体组稳定性研究"(GJJ13698)
宜春市科技支撑项目"油菜种质资源创新与利用"(JXYC2013KNA08)
关键词
甘蓝型油菜
黑芥
种间杂交
减数分裂
Brassica napus
Brassica nigra
interspecific hybrid
meiosis
