摘要
目的：建立盐酸罗哌卡因多囊脂质体中药物含量及包封率的测定方法。方法：采用低速离心法分离游离药物和多囊脂质体,采用高效液相色谱法检测多囊脂质体中游离药物含量和总药量,并计算包封率。结果：盐酸罗哌卡因在1.0~80.0μg.ml-1范围内线性关系良好（r=0.9998）；平均回收率为99.95%,RSD为0.72%（n=9）。用此方法测定3批盐酸罗哌卡因多囊脂质体的主药含量和包封率,结果分别在99.1%~100.3%及80.06%~82.14%之间。结论：该方法操作简单,结果准确,适合用于盐酸罗哌卡因多囊脂质体含量和包封率测定。
Objective：To establish a determination method for the content and entrapment efficiency of ropivacaine hydrochloride-loaded multivesicular liposomes. Methods： The separation of the multivesicular liposomes from the free drug was achieved by low-speed centrifugation. The concentration of ropivacaine hydrochloride in the supernatant and the multivesicular liposomes was determined by HPLC, and the entrapment efficiency was calculated. Results： The linear range of ropivacaine hydrochloride was 1. 0-80. 0μg. ml-1（r=0. 999 8）. The average recovery was 99. 95% and RSD was 0. 72%（n=9）. The content and entrapment efficiency of three batches of ropivacaine hydrochloride-loaded multivesicular liposomes was within the range of 99. 1%-100. 3% and 80. 06%-82. 14%, respectively. Conclusion：The method is simple and accurate, and can be used in the determination of content and entrapment efficien-cy of ropivacaine hydrochloride-loaded multivesicular liposomes.
出处
《中国药师》
CAS
2015年第1期14-17,共4页
China Pharmacist
基金
湖北省自然科学基金项目(编号:2013CFB430)
关键词
盐酸罗哌卡因
多囊脂质体
含量
包封率
高效液相色谱法
Ropivacaine hydrochloride
Multivesicular liposomes
Content
Entrapment efficiency
HPLC
