摘要
分别以D-葡萄糖、D-果糖、D-木糖、D-甘露糖、D-半乳糖、D-阿拉伯糖和L-阿拉伯糖为碳源,研究对木醋杆菌和红茶菌生产细菌纤维素的影响。碳源初始浓度分别为25 g/L和100 g/L,经过10天培养。结果表明,D-果糖是这两类微生物的最佳碳源,D-葡萄糖次之,D-阿拉伯糖的消耗则未测到。木醋杆菌和红茶菌均能利用D-木糖和D-半乳糖生产细菌纤维素,但是产量不能与D-果糖和D-葡萄糖的相比。在高浓度碳源培养基中,红茶菌比木醋杆菌有更高的消耗率,其可大量利用D-甘露糖,但主要用于菌体生长而非纤维素合成。结果发现糖源种类对于细菌纤维素的微结构影响很小。
Lignocellulosic biomass can be utilized as a carbon source after hydrolysis to produce bacterial nanocellulose （BNC）, but there are few reports of evaluation on the effect of the diverse sugars in the hydrolysates on the production of BNC. Seven different sugars including D-glucose, D-fructose, D-xylose, D-mannose, D-galactose, D-arabinose and L-arabinose were used as carbon sources to compare their effects on cellulose production by Gluconacetobacter xylinus and Kombucha （black tea fungus）. The initial concentrations of carbon source were 25 g/L and 100 g/L respectively. After 10-d cultivation, the results revealed that D-fructose and D-glucose was the best and the second best carbon source respectively for both of the microorganisms, while consumption of D-arabinose was not detected. D-xylose and D-galactose could be used to produce BNC, but the yields of BNC were much lower than those from fructose and glucose media. In the medium with high concentration carbon source, the consumption rate of sugars of Kombucha was higher than that of G. xylinus. Kombucha had its advantage in the utilization of mannose, which was mainly devoted to the growth of the microorganism instead of the production of BNC. The SEM inspection showed no remarkable morphological difference in the microfibrils of BC obtained in seven sugar-based culture media.
出处
《纤维素科学与技术》
CAS
CSCD
2014年第4期18-27,共10页
Journal of Cellulose Science and Technology
基金
国家自然科学基金(51373031)
教育部新世纪优秀人才支持计划项目(NCET-12-0828)
上海市科委项目(12nm0500600)
中央高校基本科研业务费专项资金资助项目
关键词
细菌纳米纤维素
木醋杆菌
红茶菌
碳源影响
bacterial nanocellulose
Gluconacetobacter xylinus
Kombucha
carbon source effect
