摘要
原人参二醇类皂苷混合物经人参皂苷酶生物转化后可生成F2、C-Mc等7～10种稀有人参皂苷。天然人参中不含人参皂苷C-Mc,且F2的含量极低。在生物转化所得的人参二醇类皂苷酶反应产物中,分离纯化得到稀有人参皂苷F2与C-Mc,并进行HPLC检测。反应后得到粗产品40g,经脱糖脱色和硅胶柱分离后,成功得到稀有人参皂苷F23.49g,纯度为98.2%,得率8.72%;得到C-Mc 0.70g,纯度为82.2%,得率为1.80%。成功分离出了F2和C-Mc制品,建立了初步的分离制备方法。
Protopanaxadiol ginsenosides were transformed into 7-10 types of rare ginsenosides such as F2 and C-Mc by the ginsenoside enzyme.F2 has a very low content in the natural and C-Mc does not exist in natural ginseng.The crude product transformed from protopanaxadiol ginsenosides（PPD）was separated and purified.Silica gel chromatography was applied to separate and purify 40 g crude product,which has been desugared and decolored.3.49 g ginsenoside F2 with the purity of 98.2%was obtained,and its yield was 8.72%.0.70 g C-Mc with the purity of 82.2% was also obtained,and its yield was 1.80%.This experiment has successfully established a preliminary method of preparing and separating on F2 and C-Mc.
出处
《大连工业大学学报》
CAS
北大核心
2014年第6期420-422,共3页
Journal of Dalian Polytechnic University
基金
"重大新药创新"科技重大专项(2012ZX09503001-003)
