摘要
采用苯酚-硫酸法对柳芽中多糖含量按紫外分光光度法在482nm波长处测定吸光度,实验结果表明葡萄糖对照品在5.4~16.2μg/ml之间呈线性关系,该法简便易行,准确性高,重现性好。本文探讨了测定过程的影响因素,并通过此法测得柳芽中多糖含量为7.46%。
The content ofpolysaccharides in willow sprouts was determined by the method of phenol-sulfuric acid. The results showed a good linearity relationship between the absorbance and the content of standard glucose in the range of 5.4~ 16.2μg/ml. The method is simple, convenient and accurate, and has good reproducibility. The affecting factors in the assaying process were discussed in this paper respectively. By this method, the content of polysaccharides in willow sprouts was found to be 7.46% o
出处
《中国西部科技》
2014年第12期64-65,共2页
Science and Technology of West China
基金
山东省医药卫生重点实验室培养基金项目
关键词
柳芽
多糖
苯酚-硫酸法
含量
Willow Sprouts
Polysaccharides
Phenol-Sulfuric Acid Method
