摘要
目的:探讨结核蛋白芯片检测在结核病诊断中的价值。方法:使用蛋白芯片系统检测血清结核抗体的病例共4 502例,其中结核病例464例,非结核病例4 038例。我们采用蛋白芯片技术检测结核杆菌中5种成分,即ESAT-6、CFP10、LAM、38 000融合蛋白和16 000融合蛋白,分析其结果。结果:464例结核病例中共323例结核抗体阳性,4 038例非结核病例中788例阳性,结核蛋白芯片检测的灵敏度为69.61%,特异度为80.49%。结论:结核蛋白芯片是诊断结核病的较有效方法,具有特异性好、快速方便等优点,对结核病快速辅助诊断有积极作用。但对结核病的疗效监测无实用价值。
Objective： To explore the diagnosis value of tuberculosis protein chip for tuberculosis.Methods： The antibody levels of tuberculosis protein ESAT- 6,CFP10,16 000,38 000 and LAM in 464 cases with tuberculosis and 4038 cases with non- TB were detected with protein chip. And there were the analysis and statistics. Results： The tuberculosis antibody positive were detected as 323 of 464 cases with tuberculosis and 788 of 4038 cases with non- TB. The sensitivity of tuberculosis protein chip was 69. 61%,and the specificity was 80. 49%. Conclusion： The tuberculosis protein chip is a fast,easy and effective method for diagnosing the tuberculosis,and has good specificity for the rapid diagnosis of the tuberculosis,and there is not practical in monitoring the effects of treating tuberculosis.
出处
《现代临床医学》
2014年第6期414-415,418,共3页
Journal of Modern Clinical Medicine
基金
攀枝花市应用技术研究与开发项目(项目编号:2013CY-S-16-10)
关键词
结核分枝杆菌
蛋白芯片
抗体
mycobacterium tuberculosis
protein chip
antibody
