摘要
采用反相高效液相色谱（ RP-HPLC）法检测五味子中三种木脂素类成分的含量。色谱柱为Agilent C18色谱柱（250 mm ×4.6 mm，5μm），流动相为甲醇-水（75：25），流速1.0 mL/min，检测波长250 nm，柱温30℃，进样量10μL。结果表明，3种成分的进样量分别在4~800、4~400、4~400μg/mL范围与各自峰面积积分值呈良好的线性关系（ r 分别为0.9993，0.9998，0.9999），平均回收率分别为98.44%，98.86%和97.84%，RSD分别为1.24%，1.87%和1.18%（n 均为5）。北五味子中五味子醇甲、五味子甲素和五味子乙素含量分别为7.196，1.344，3.476 mg/g，南五味子中不含五味子醇甲，五味子甲素、五味子乙素的含量依次为59.065，3.213 mg/g。该方法快速、准确、重现性好，可用于南、北五味子中木脂素类成分的分析，评价不同来源五味子的质量。
Three lignans in Schisandra Chinensis（ Turcz. ）Baill. were determined by RP-HPLC method. The separation was performed on an Agilent C18（250 mm × 4. 6 mm,5 μm）with UV detection at 250 nm,the mobile phase consisted of methanol-water（75：25）,the flow rate was 1. 0 mL/min and the columm temperature was controlled at 30 ℃,the feeding amount is 10 μL. Results showed the linear range of Schisandrin,Deoxyschizandrin and Schizandrin B were 4 ~800 μg/mL（ r =0. 999 3 ）,4 ~400 μg/mL（r=0. 999 8）,4~400 μg/mL（r=0. 999 9）,respectively. The mean recovery of three com-ponents were 98 . 44%（ RSD =1 . 24%,n =5 ）,98 . 86%（ RSD =1 . 87%,n =5 ）,97 . 84%（ RSD =1. 18%,n=5）,respectively. The contents of Schisandrin,Deoxyschizandrin and Schizandrin B in Schi-zandra sinensis Baill. are 7. 196,1. 344,3. 476 mg/g,respectively. The contents of Deoxyschizandrin and Schizandrin B in Schisandra sphenanthera Rehd. et Wils are 59. 065,3. 213 mg/g,respectively. The meth-od is quick,accurate,reproducible and can be used to analyze lignans between Schisandra sphenanthera and Schizandra sinensis Baill. as well as evaluate their quality.
出处
《应用化工》
CAS
CSCD
2014年第11期2107-2110,共4页
Applied Chemical Industry
基金
陕西省科技统筹创新工程计划项目(2011KTCL03-03)
西安科技局项目生物医药新产品新技术开发项目(CXY1348(5))
西安医学院2012年校级科研计划扶植项目(12FZ02)
