摘要
本试验对野生虎斑乌贼5种组织（肌肉、肝脏、卵巢、缠卵腺、副缠卵腺）的营养成分进行分析,旨在对其营养价值进行评估。随机选取雌、雄野生虎斑乌贼[雌性个体平均体长（31.37±0.32） cm,平均体重（2173.33±141.10） g；雄性个体平均体长（30.97±0.74） cm,平均体重（2476.33±117.54） g]各10尾,采用生化分析手段对其肌肉、肝脏、卵巢、缠卵腺、副缠卵腺中水分、粗灰分、粗蛋白质、粗脂肪含量以及氨基酸、脂肪酸组成与含量进行测定与分析,每个组织测定3个平行。结果表明：野生虎斑乌贼5种组织中,水分含量为60.12%-78.33%,各组织间差异显著（P〈0.05）；粗蛋白质含量为31.47%-77.93%,表现为肌肉（77.93%）〉卵巢（62.39%）〉副缠卵腺（58.62%）〉缠卵腺（57.64%）〉肝脏（31.47%）,各组织间差异显著（P〈0.05）；粗灰分含量为1.26%-3.18%,除肌肉与缠卵腺无显著差异（ P〉0.05）外,其他组织间差异显著（ P〈0.05）,以副缠卵腺最高,肝脏最低；粗脂肪含量为2.46%-12.82%,表现为肝脏（12.82%）〉缠卵腺（4.80%）〉副缠卵腺（3.42%）〉肌肉（3.14%）〉卵巢（2.46%）,除肌肉与副缠卵腺无显著差异（P〉0.05）外,其他组织间差异显著（ P〈0.05）。野生虎斑乌贼5种组织均含有17种氨基酸,其中总氨基酸（ TAA）含量为27.68%-77.87%,必需氨基酸（ EAA）含量为13.60%-31.80%；共检出26种脂肪酸,包括12种饱和脂肪酸（ SFA）,7种单不饱和脂肪酸（ MUFA）和7种多不饱和脂肪酸（PUFA）,其中PUFA含量为27.48%-54.15%,以肝脏最低,显著低于其他组织（P〈0.05）,具体为缠卵腺（54.15%）〉肌肉（47.74%）〉卵巢（45.50%）〉副缠卵腺（41.49%）〉肝脏（27.48%）；C20∶5n-3（ EPA）含量为6.72%-16.03%,C22∶6n-3（ DHA）含量为12.79%-32.20%。由此得出,野生虎斑乌贼是一种高蛋白质、低脂肪、氨基酸含量高且种类齐全、PUFA含量丰富（尤
In order to evaluate the nutritive value of wild Sepia pharaonis, the nutritional components of differ-ent tissues （ muscle, liver, ovary, nidamental gland and accessory nidamental gland） were determined. Ten fe-male and 10 male wild Sepia pharaonis were randomly selected [ female individual： average body length （31.37±0.32） cm, average body weight （2 173.33±141.10） g; male individual： average body length （30.97±0.74） cm, average body weight （2 476.33±117.54） g]. The contents of moisture, ash, crude pro-tein, crude fat, and the composition and contents of amino acids and fatty acids in muscle, liver, ovary, nida-mental gland and accessory nidamental gland were determined by biochemical analysis methods. Each organiza-tion was measured with 3 replications. The results showed that moisture and crude protein contents were signifi-cantly different among different tissues （P〈0.05）. The moisture content in 5 tissues of wild Sepia pharaonis was 60.12% to 78.33%. The crude protein content in 5 tissues of wild Sepia pharaonis was 31. 47% to 77.93%, with the highest value in muscle （77.93%）, followed by ovary （62.39%）, accessory nidamental gland （58.62%）, nidamental gland （57.64%） and liver （31.47%）. The ash content in 5 tissues of wild Sepia pharaonis was 1.26% to 3.18%, and there was a significant difference （P〈0.05） with exception of muscle and nidamental gland （ P〉0.05） , with the highest value in accessory nidamental gland and the lowest value in liver. The crude fat content in 5 tissues of wild Sepia pharaonis was 2.46% to 12.82%, and there was a signifi-cant difference （ P〈0.05） with exception of muscle and accessory nidamental gland （ P〉0.05） . The liver had the highest crude fat content （12.82%）, followed by nidamental gland （4.80%）, accessory nidamental gland （3.42%）, muscle （3.14%） and ovary （2.46%）. A total of 17 amino acids were identified in 5 tissues of wild Sepia pharaonis. The total amino acid content in 5 tis
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第12期3858-3867,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(41206114)
宁波市农业科技专项(201401C1111001)
宁波市科技重点项目(2011C11002)
关键词
野生虎斑乌贼
组织
营养成分
评价
wild Sepia pharaonis
tissue
nutritional components
evaluation
