摘要
为了解凡纳滨对虾(Litopenaeus vannamei)在选育中间养成阶段(10~40 g/尾)的营养需求,以同一全同胞家系体质量为(10.30±1.61)g的凡纳滨对虾为对象,选择配合饲料、软颗粒饲料、虎斑乌贼(Sepia pharaonis)和双齿围沙蚕(Perineresis aibuhitensis)这4种饵料进行60 d的投喂,在对4种饵料进行营养评价的基础上,研究其对对虾生长、成活以及消化、生长相关基因表达的影响。结果显示:4种饵料投喂下对虾的特定生长率和成活率从高到低依次为双齿围沙蚕、配合饲料、软颗粒饲料和虎斑乌贼;人工饲料组对虾肝胰腺中消化相关的α-淀粉酶d4基因表达水平显著高于生物饵料组,蛋白酶基因表达趋势同α-淀粉酶d4基因相似,双齿围沙蚕组和软颗粒饲料组的胰脂肪酶基因表达显著高于配合饲料组。虎斑乌贼组小核核糖核蛋白肽G基因表达显著低于其他饵料组,生物饵料组y+L氨基酸转运载体-2基因表达显著高于人工饲料组;虎斑乌贼和双齿围沙蚕的氨基酸营养评价相对较高,而配合饲料和双齿围沙蚕的脂肪组成和含量相对更优。研究发现:双齿围沙蚕的营养价值最高,软颗粒饲料最低;4种实验饵料的投喂效果依次为双齿围沙蚕、配合饲料、软颗粒饲料和虎斑乌贼;除蛋白酶基因外,其他基因表达同实验饵料的营养组成和投喂效果一致。
In order to know the nutrition demands of Litopenaeus vannamei during the middle stage of selective breeding preservation period, juvenile shrimps [(10.30±1.61) g/ind.] from the same breeding family were fed with two artificial diets [formula feed(ZD) or soft pellet feed(YW)] or two live diets [Sepia pharaonis(SP) or Perineresis aibuhitensis(PA)] for 60 days. The nutrition composition of these four diets was determined and the effects of different diets on the growth performance, survival rate and the expression levels of digestion and growth-related genes were evaluated. The results showed that: shrimps which were fed with PA had the highest specific growth rate and survival rate, followed by ZD, YW, and SP;expression levels of α-amylase d4 and trypsin in hepatopancreas of shrimps fed with artificial diets were significantly higher than those fed with live diets;the expression level of pancreatic lipase in shrimps fed with PA or YW was significantly higher than that of formula feed. Shrimps fed with SP had significantly lower expression level of small nuclear ribonucleoprotein polypeptide G(SNRPG) than others, whereas shrimps fed with live diets had significant higher expression level of y+L amino acid transporter 2 than those fed with artificial diets. the PA and SP had relatively higher amino acid evaluation scores, while ZD and PA possessed optimal fatty acid composition and content. In conclusion, PA and YW had the highest and lowest nutritional values, respectively;among the four diets, shrimps fed with PA had the best growth performance, and the expression patterns of digestion or growth-related genes were consistent with the nutrition composition(except trypsin) of the diets.
作者
冀德伟
张敏
闫茂仓
柴雪良
胡利华
JI Dewei;ZHANG Min;YAN Maocang;CHAI Xueliang;HU Lihua(Zhejiang Mariculture Research Institute,Wenzhou 325005,Zhejiang,China;Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Wenzhou 325005,Zhejiang,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2021年第2期266-275,共10页
Journal of Shanghai Ocean University
基金
浙江省重大科技专项(2016C02055-5)
浙江省省属科研院所专项(2017F30041)
浙江农业新品种选育重大科技专项(2016C02055-5)
温州市农业新品种选育协作组项目(2019ZX002-01)
关键词
凡纳滨对虾
选育
中间养成
饵料
生长
基因表达
Litopenaeus vannamei
selective breeding
middle stage
diet
growth
gene expression
