摘要
对不同细胞进行牛血清中牛源病毒检测的比较实验,选出用于牛血清中病毒检测的最适合外源病毒增殖的细胞,从而提高血清中病毒的检出率,以确保用于疫苗生产的牛血清安全可靠。将牛腹泻病毒、牛腺病毒、牛细小病毒、牛副流感病毒、呼肠孤病毒和狂犬病毒接种到培养好的MDBK细胞、VERO细胞、BT细胞中,逐日观察细胞的病变情况,待观察到10%细胞出现病变或者培养7 d后,进行免疫荧光染色,选出适合用于六种病毒增殖的细胞系。结果显示,BT细胞对所有病毒敏感,VERO细胞对BAV、BVDV病毒不敏感,MDBK对REO、BVDV、RV三种病毒不敏感。综合比较后,选择BT细胞为BPIV、BPV、BAV、BVDV的增殖及实验用细胞,VERO细胞为REO、RV的增殖及实验用细胞。
Choose the most suitable cell that was used in testing for exogenous virus in bovine serum, then as sensitive cells for detecting the virus in bovine serum in order to improve the detection rate of virus in the serum, it ensured that the bovine serum which was used in production of vaccine was safe. Be ready to cultivate appropriate cells including MDBK, VERO, BT. At the same time,six kinds of virus referred to the BPV, BVDV, BAV, BPIV, REO, RV, Six kinds of virus was inoculated on these three cells. Observing the cells which was inoculated virus every day. After 10% cell was appeared lesion or the cell was cultivated for 7 days, take out the cell, and these cells were dyeing by immunofluorescence, then according to the staining results to determine which cell was suitable for detecting these virus. The results showed that BT cell was sensitive to all cells, Vero cell was not sensitive to BAV, BVDV, MDBK was not sensitive to REO, BVDV, RV. The results of this study showed that BT cells can be used in proliferation and testing for BPV, BPIV, BAV, BVDV, and Vero cell can be used for REO, RV. In order to improve the detection rate of virus in the serum, we can choose a variety of cells at the same time.
出处
《中国兽药杂志》
2014年第12期38-42,共5页
Chinese Journal of Veterinary Drug
关键词
细胞
病毒
增殖
cell
virus
proliferation
