摘要
目的:研究有氧运动对大鼠动脉粥样硬化(AS)发生发展中,血浆中一氧化碳(CO)和环磷酸鸟苷(c GMP)的变化,寻找CO发挥作用的机制,为有氧运动预防动脉粥样硬化发生发展提供理论依据。方法:选Sprague-Dawley健康雄性大鼠30只随机分为3组,正常对照组(CG);高脂肪膳食致动脉粥样硬化组(ASG);高脂肪膳食+60min分钟有氧运动组(EG),每组各10只,EG组有氧运动8周。检测8周前后各组血脂TC、TG、LDL、HDL、VLDL指标,及血浆中CO和环磷酸鸟苷c GMP含量,显微观察主动脉切片组织形态学变化。结果:EG组TC、TG、LDL、VLDL较ASG组明显降低(P<0.05),HDL较ASG组明显升高(P<0.05)。EG组可见内膜细胞增生、灶性脱落,中膜平滑肌细胞排列相比ASG组有规律,平整有光泽。平滑肌细胞增生、钙化范围及程度明显少于ASG组。ASG组血浆中CO和c GMP含量明显高于CG组(P<0.05),而EG血浆中CO和c GMP含量明显低于ASG组。血浆中CO的变化和c GMP的变化成正相关(r=0.84)。结论:在高脂肪膳食致动脉粥样硬化发生发展中,血浆中CO和c GMP含量升高,有氧运动可降低升高的CO和c GMP含量;CO-c GMP结合途径可能是CO发挥效应的机制之一。
Objective：the paper is to explore the effects of Aerobic-exercise（ swimming） on CO（ Carbon Monox-ide） and cGMP（Cyclic Guanosine Monophosphate）of the rat’s plasma during the development of atherosclerosis（AS） and to investigate the changes and correlations of CO and cGMP for looking for the possible mechanisms that CO works so as to provide a theoretical basis for Aerobic-exercise Preventing atherosclerosis development. Methods：30 Sprague-Dawley healthy male rats are randomly divided into 3 × 10 groups for 8 weeks of experiment, the control group（CG）, the atherosclerosis group（ASG） with high -fat diet and the Aerobic -exercise group（AEG） in which the rats swim 60min every day with high-fat diet. The contents of CO and cGMP of the plasma are measured after and before experi-ment. Results：The contents of CO and cGMP of the ASG are significantly increased（P〈0. 05. The contents of CO and cGMP of the AEG are significantly lower than those in the ASG（P〈0. 05. The changes of CO in the plasma are posi-tively correlated with the changes of cGMP （ r=0 . 84 ） . Conclusion： the contents of CO and cGMP in plasma increase during the development of atherosclerosis. Aerobic-exercise may reduce the contents of CO and cGMP in plasma. CO-cGMP pattern may be one of the mechanisms that CO works.
出处
《成都体育学院学报》
CSSCI
北大核心
2014年第12期91-94,共4页
Journal of Chengdu Sport University
