摘要
研究60Coγ-射线不同剂量辐照落花生茎叶提取生物碱的工艺条件及参数,并分析落花生茎叶生物碱的种类。采用紫外分光光度法测定总生物碱的含量或吸光值,考察大孔树脂种类、型号,洗脱液的种类、浓度等对落花生茎叶总生物碱纯化效果的影响,并用液相色谱对落花生茎叶生物碱进行定性定量分析。结果显示,总生物碱含量随吸收剂量的增加,先上升后下降;在吸收剂量为5 k Gy时达到最高(7.970±0.117)%,显著优于超声波提取法的(1.485±0.061)%(t检验,p<0.001)。大孔树脂AB-8的纯化效果最好,洗脱溶媒为60%甲醇时洗脱效果最好,获得的生物碱纯度可达99.99%。高效液相色谱结果分析表明,落花生茎叶生物碱主要为胆碱,其含量约为2.61 g·kg-1花生茎叶。
The aim is to obtain process conditions and parameters of total alkaloids from Arachis hypogaea L. stem and leaf （AHSL） by the irradiation extraction and macroporous resin purification. Total alkaloids of AHSL were extracted by Co-60 γ-ray irradiation at different doses. Total alkaloid content or absorbance value was measured by ultraviolet spectrophotometry. Effects of macroporous resin type and model, and the eluent type and concentration were investigated by static and dynamic adsorption examine. Qualitative and quantitative analysis of AHSL alkaloid were carried out by high performance liquid chromatography. The results showed that a trend of rise first and then fall was observed in total alkaloid content, with the increase of irradiation dose. The highest alkaloid yield was （7.970±0.117）% at irradiation dose of 5 kGy in Co-60 γ-ray irradiation method, which is better than that （1.485±0.061）% in ultrasonic method （t test, p〈0.001）. The alkaloids purity can reach 99.99% using AB-8 macroporous resin and elution solvent at 60% methanol elution. In HPLC results, it was found that AHSL alkaloids mainly is choline, and the yield is about 2.61 g.kg^-1AHSL.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2014年第6期45-50,共6页
Journal of Radiation Research and Radiation Processing
基金
湖北省自然科学基金(2012FFB01403)
湖北省科技型中小企业技术创新计划(CXW2014000249)
公益性行业(农业)科研专项经费(201103007)资助
关键词
落花生茎叶
^60CO
γ-射线
辐照
生物碱
提取
分析
Arachis hypogaea L. stems and leaves, Co-60 γ-rays, Irradiation, Alkaloids, Extraction, Analysis CLC 0629.3, TL99
