摘要
目的：建立复方仙灵脾颗粒的质量控制方法。方法：采用TLC法对方中淫羊藿、枸杞子进行鉴别;采用HPLC法测定样品中淫羊藿苷的含量。色谱条件：色谱柱为Welchrom C18柱（200 mm×4.6 mm,5μm）;流动相：乙腈-水（25∶75）;流速为1.0 m L/min;检测波长：270 nm;柱温：30℃。结果：薄层定性鉴别的斑点清晰,分离效果良好;淫羊藿苷含量在3~160μg/m L范围内线性关系良好（r=0.9998）,平均加样回收率为100.20%,RSD为2.55%（n=6）。结论：本方法结果准确、操作简单,重复性良好,适合作为该制剂的质量控制标准。
Objective ： To establish a quality standard for Fufang Xianlingpi Granule. Methods ： Epimedii folium and Lycii fructus were identified by TLC method. The content of ieariin in Fufang Xianlingpi Granule was determined by HPLC. The Welehrom C18 （200 mm×4.6 mm,5μm）column was used. The mobile phase consisted of acetonitrile-water （ 25 ： 75 ） at a flow rate of 1.0 mL/min. The detection wavelength was at 270 nm under 30 ℃ . Results ： The spots in the thin layer chromatography were clear. The icariin showed a good linear relationship within the range of 3-160 μg/mL （r=0.9998）. The average recovery was 100.20%, and the RSD was 2.55%. Conclusion ： The method is simple, accurate and repeatable, which can be used for quality control of Fufang Xianlingpi Granule.
出处
《辽宁中医药大学学报》
CAS
2014年第12期51-53,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
军队医疗机构制剂标准提高科研专项课题(13ZJZ02)
