摘要
研究大肠杆菌表达的水稻α-半乳糖苷酶基因工程菌菌体破碎的条件。以菌液酶活性为指标,用超声波破碎、溶菌酶降解及菌体反复冻融3种方法破碎细胞。结果表明:超声波功率为200 W、破碎时间为1 min时测得的菌液酶的活性最大,为7.757 7U/m L,细胞破碎效果最好;菌体反复冻融条件下测得的菌液酶活性随冻融次数的增加而增大,但样品处理时间过长;菌液酶活性随加入溶菌酶浓度增加而增加,但有处理时间长、用量大、细胞破碎不完全的缺点。
The research studies the E.coli expressed rice.It studied cell disruption conditions of α-Galactosidase Gene Engineering.It used 3 methods for the examination：ultrasonic-break,lysozyme treatment,and thalli repeated freeze-thaw to disrupt cells,using liquid enzyme activity as indicator.The results showed that the optimal disruption is ultrasonic-breaking at power 200 W for 1 min and the α-galactosidase activity was yield at 7.757 7 U/m L;under the condition of thalli repeated freeze-thaw,the liquid enzyme activity increased with the number of freeze-thaw increasing,but the treatment time is too long;similarly,the α-galactosidase activity was enhanced by a rise of the lysozyme concentration and prolongation of treatment time,whereas,it was limited by longer treating time,higher lysozyme dosage and incomplete cell break.
出处
《农业科技与装备》
2014年第10期37-39,共3页
Agricultural Science & Technology and Equipment
基金
辽宁省大学生创新创业训练计划(201310166037)
