摘要
以1 000 m L烧杯为容器,木葡糖醋杆菌在静置培养时,培养液体积为100 m L时,没有变异;增至300 m L时,发生变异,纤维素产量下降38.8%,培养液体积增至700 m L时,变异菌数量高达1.3×106 CFU/m L,纤维素产量下降76.3%;120 r/min振荡培养更易发生变异,两种方式培养下的变异现象通过筛网使用而得到控制,从而提高了纤维素产量。
U sing 1 000 m lbeakerascontainer,understationary cultivation ofG luconacetobacterxylinus,m utation didn’toccurw hen the loading vol-um e w as100 m l.H ow ever,m utation ofG a.xylinusoccurred understationary cultivation in 300 m lm edium ,w hich led to decrease ofbacterialcellu-lose （B C ）yield by 38.8% .W hen the m edium volum e increased to 700 m l,B C yield decreased 76.3% ,and the m utantbacterialnum berw as 1.3×10^6 C FU /m l.C om pared w ith static cultivation,m utation ratesofG a.xylinusw ere higher,w hen shaking at120 r/m in.M utation w asinhibited by m esh fil-terno m atterw hich cultivation m ethod w asused.Thereby the cellulose production w asincreased.
出处
《中国酿造》
CAS
2014年第11期67-69,共3页
China Brewing
基金
海南省重大科技项目(ZDZX2013011)
关键词
木葡糖醋杆菌
变异
筛网
G luconacetobacterxylinus
m utation
m esh filter
