摘要
18种氨基酸被作为唯一氮源添加到木醋杆菌合成培养基中检验其对纤维素产量的影响。根据产纤维素的情况将氨基酸分为产量较多类、产量较少类及不产类,再通过正交试验检验产量较多类及较少类氨基酸的组合效应。结果表明,只有组氨酸适合于作为氮源物质添加到木醋杆菌合成培养基中,但组氨酸的效果不及硫酸铵的效果显著,通过定量测定组氨酸及硫酸铵在培养基中的含量变化,表明二者共同存在时,都被木醋杆菌利用,利用率并不互相影响。
A total of 18 amino acids were added respectively into the defined medium for Acetobacter xylinum to determine their effect on the cellulose yield.Based on the production of cellulose,these amino acids were divided into three categories： high-yield（7）,low-yield（9） and zero-yield（2）.In addition,the respective formulation of 7 high-yield and 9 low-yield amino acids was investigated using orthogonal array design.It was found that histidine was the optimum nitrogen source in the defined medium among these 18 amino acids,but inferior to ammonium sulphate.Coexisting histidine and ammonium sulphate could be utilized without undesirable interaction.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第9期204-207,共4页
Food Science
基金
国家公益性行业(农业)科研专项(200903026-6)
关键词
氨基酸
木醋杆菌
合成培养基
amino acids
Acetobacter xylinum
defined medium
