摘要
目的观察金黄色葡萄球菌感染人巨噬细胞系U937细胞后信号通路Toll样受体4(TLR4)-p38蛋白激酶(p38MAPK)的表达及意义。方法体外培养人巨噬细胞系U937细胞,感染0、30、60和90 min时收集细胞,应用Western blot法检测各组TLR4和p38MAPK蛋白表达的变化;在另一实验中,分为对照组、金黄色葡萄球菌感染60 min组及p38MAPK抑制剂SB203580 5 mg/m L干预组、SB203580 10 mg/m L干预组,应用Western blot法检测各组TLR4和p38MAPK蛋白表达的变化。结果随着感染时间的延长,TLR4和p38MAPK蛋白的表达逐渐增加;给予SB203580抑制剂后,TLR4和p38MAPK蛋白的表达明显减弱。结论金黄色葡萄球菌感染U937细胞可引起TLR4-p38MAPK信号通路的活化,而SB203580对其有明显的抑制作用,证明TLR4-p38MAPK信号通路与金黄色葡萄球菌感染U937细胞密切相关。
Objective To investigate the effect and significance of TLR4 and p38MAPK signaling pathway expressed in human monocytic U937 cells infected by Staphylococcus aureus. Methods U937 cells were infected by Staphylococcus aureus were collected at 0,30,60 and 90 min after infection respectively,and Western blot was per-formed to detect the expressions of TLR4 and p38MAPK. In another experiment,the routinely cultured U937 cells in vitro were divided into control group,Staphylococcus aureus stimulation group,SB203580（5 mg/mL or 10 mg/mL） plus Staphylococcus aureus treatment groups. Western blot was performed to detect the expressions of TLR4 and p38MAPK. Results The expressions of TLR4 and p38MAPK increased in a time-dependent manner. Compared with those in the control group,the expressions of TLR4 and p38MAPK increased more significantly in U937 cells induced by Staphylococcus aureus. SB203580 could effectively inhibit the expressions of TLR4 and p38MAPK. Conclusion The TLR4-p38MAPK signaling pathway can be activated by Staphylococcus aureus in U937 cells,and SB203580 sig-nificantly inhibits the signaling pathway,demonstrating the close relation between the TLR4-p38MAPK signaling path-way in Staphylococcus aureus-infected U937 cells.
出处
《实用药物与临床》
CAS
2014年第11期1371-1373,共3页
Practical Pharmacy and Clinical Remedies
基金
国家自然科学基金资助项目(81101224)
