摘要
目的利用脂多糖刺激家蝇胚胎细胞产生并提取抗菌蛋白,对比研究家蝇胚胎细胞抗菌蛋白和高三尖杉酯碱(HHT)对人髓样白血病细胞K562和正常人体细胞的抑制杀伤作用。方法采用含20mg/L脂多糖的无血清M3培养基作用于处于对数生长期的家蝇胚胎细胞16h,提取上清液中的抗菌蛋白,将抗菌蛋白配制为40、80、160、320和640μg/mL 5个浓度组,采用MTT法测定各浓度抗菌蛋白对K562和人脐静脉血管内皮细胞的抑制作用。将处于对数生长期的K562细胞和人脐静脉血管内皮细胞配制成相同浓度的HHT组、家蝇胚胎细胞抗菌蛋白组,两种细胞均采用自身设置对照组。采用流式细胞术测定K562细胞和人脐静脉血管内皮细胞3组药物的有效杀伤率。结果 MTT法检测显示,5种浓度抗菌蛋白对K562细胞均具有明显抑制作用,各浓度抗菌蛋白对人脐静脉血管内皮细胞均无抑制作用。对照组、HHT组及家蝇胚胎细胞抗菌蛋白组对K562细胞的有效杀伤率分别为(28.16±2.14)%、(81.41±1.95)%和(82.90±3.03)%;对人脐静脉血管内皮细胞的有效杀伤率分别为(41.13±2.51)%、(82.20±2.57)%和(36.68±1.86)%。结论家蝇胚胎细胞抗菌肽与常用的白血病化疗药物相比,优点在于能有效杀伤白血病细胞又不损伤正常人体细胞。
Objective To stimulate the embryonic cells of the musca domestica with lipopolysaccharide（LPS） for production of antibacterial protein and to extract antibacterial protein ,then research the inhibitory action of the antibacterial protein and homohar‐ringtonine on human myeloid leukemia cells K562 and normal human cells .Methods The logarithmic growth phase′s embryonic cells of the musca domestica were stimulated using no serum M3 insect medium which contained 20 mg/L LPS for sixteen hours . The antibacterial protein was extracted from supernatant fluid .The antibacterial protein was prepared in 40 ,80 ,160 ,320 and 640μg/mL five density groups ;the MTT experiments were used to test the inhibition of antibacterial protein on K562 cells and the hu‐man umbilical vein vascular endothelial cells .The K562 cells and human umbilical vein vascular endothelial cells were prepared HTT and antibacterial protein of the embryonic cells of the musca domestica groups ,the normal control group was established by cells itself .Effective killing rate of K562 cells and the human umbilical vein vascular endothelial cells were measured .Results The effective inhibition ratio of homoharringtonine and the antibacterial protein on K562 cells and human umbilical vein vascular endo‐thelial cells on three density groups were detected by flow cytometry .The MTT examination demonstrated that all density antibac‐terial peptides had inhibition activities on K562 cells ,but no inhibition activities on human umbilical vein vascular endothelial cell . The effective killing and wound ratio of the control group ,the homoharringtonine group and of the antibacterial protein group from the embryonic cells of the musca domestica on the K562 cells were（28 .16 ± 2 .14）% ,（81 .41 ± 1 .95）% and （82 .90 ± 3 .03）% ,re‐spectively ;the effective killing rate on human umbilical vein vascular endothelial cells were（41 .13 ± 2 .51）% ,（82 .20 ± 2 .57）% and （36 .68 ± 1 .86）% ,respectively .Conclusion Compared with the
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第33期4444-4446,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(30972749)
关键词
家蝇胚胎细胞
抗菌肽
人髓样白血病细胞
抗癌活性
高三尖杉酯碱
embryonic cells of the musca domestica
antibacterical peptide
human myeloid leukemia cells
human umbilical vein vascular endothelial cell
anti tumour activity
homoharringtonine
