摘要
目的 探讨micro RNA-122（miRNA-122）的SNP位点与肝细胞癌（HCC）遗传易感性以及术后早期复发的关系.方法 采用病例-对照研究设计,提取173例HCC患者石蜡切片组织DNA,PCR扩增,查找miRNA-122包括编码基因在内共357个碱基序列内的SNP位点,分析其与HCC易感性及术后早期复发的关系.结果 上述miRNA-122序列中只找到rs17669T＞C位点,基因库中标注的其他7个SNP位点均未发现.rs17669各基因型分布频率：C/C型7例（4.0％）、T/T型110例（63.6％）、C/T型56例（32.4％）.C/C基因型相对于T/T基因型为肝癌的保护因素（OR=0.213,95％ CI：0.062-0.732）;rs17669各基因型与术后复发无关.结论 rs17669可能与有乙肝背景的江西汉族人群肝癌遗传易感性相关,与术后复发无关.
Objective To investigate the association of single nucleotide polymorphism （SNP） in micro RNA-122 （miRNA-122） with genetic predisposition and early recurrence after resection for hepatocellular carcinoma（HCC）.Methods This is a case-control study involving 173 HCC patients.DNA were exacted from cancer tissues embedded in paraffin and were amplified by PCR.The study aimed to explore SNP in gene sequence of miRNA-122 （357 base pair including extron.The outcomes of genetic predisposition were analyzed with early recurrence after resection for HCC.Results Only rs17669 was found in miRNA-122.The genetype frequence of C/C,T/T and C/T at rs17669 gene locus were 7（4.0％）,110（63.6％）and 56（32.4％）,respectively.When compared to T/T genetype,C/C genetype was a protective factor of risk of HCC （OR =0.213,95％ CI：0.062-0.732）.Genetypes had no relationship with early recurrence after resection.Conclusion For HCC recurrence,rs17669 may be associated with genetic predisposition of HCC in Hans in Jiangxi infected with HBV.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2014年第11期786-789,共4页
Chinese Journal of Hepatobiliary Surgery
基金
江西省卫计委资助（20141121）
