摘要
为了探讨没食子酸对铜绿微囊藻的抑制效应及超微结构的影响。本文采用不同浓度的没食子酸水溶液对铜绿微囊藻(Microcystic aeruginosa)藻液进行胁迫处理,随着没食子酸浓度的增加和处理时间的延长,铜绿微囊藻的生长受到显著抑制。采用扫描电镜和透射电镜观察,处理72 h的铜绿微囊藻细胞超微结构出现不同程度改变,与对照相比,铜绿微囊藻细胞壁呈现不同程度的皱缩、塌陷、破裂,胞质中类囊体光合片层出现不同程度的分散、断裂,依附于类囊体的藻胆体减少或分散到胞质中,胞质中脂质颗粒和藻青素颗粒数量出现不同程度的增多,拟核区出现空洞,直至大部分藻细胞死亡。结果表明,没食子酸能够有效抑制铜绿微囊藻的生长,具有明显的化感作用。
We studied the inhibitory effect of gallic acid on Microcystis aeruginosaand the effect of Gallicacid on ultrastructure of M.aeruginosa.M.aeruginosa was stressed with different concentrations of Gallic acid.With the increase of both concentration and time of Gallic acid,the growth of M.aeruginosa was significantly reduced.We observed cellular ultrastructure by SEM and TEM.The cells ultrastructure of M.aeruginosa after 72 h showed the changes,compared with the control,the cells walls of M.aeruginosa became wrinkled,collapsed and ruptured.Cytoplasm thylakoids photosynthetic lamellae showed dispersion and fracture.The phycobilisomes attached to the thylakoids decreased or dispersed to the cytoplasm,the number of the lipid particles and the cyanophycin particles of cytoplasm emerged different degrees increasing,a large void appeared in the nuclear area until most of the algal cells died.Therefore,gallic acid with obvious allelopathy can effectively inhibit the growth of M.aeruginosa.
出处
《植物研究》
CAS
CSCD
北大核心
2014年第6期840-844,共5页
Bulletin of Botanical Research
基金
国家自然科学基金项目(31100401)
关键词
没食子酸
铜绿微囊藻
化感作用
超微结构
Gallic acid
Microcystis aeruginosa
allelopathy
ultrastucture
