摘要
目的探讨流感病毒对环氧合酶2(COX-2)表达的影响及其调节机制。方法流感病毒感染A549细胞,将其非结构蛋白NS1真核表达载体与COX-2基因启动子的报告质粒p COX-2-Luc共转染A549细胞,测定其荧光素酶活性,采用反转录PCR和Western印迹法检测COX-2 mRNA和蛋白表达的变化。结果流感病毒上调mRNA和蛋白的表达,其NS1蛋白能显著激活COX-2基因启动子的活性,并上调COX-2 mRNA和蛋白的表达,激活作用呈剂量依赖关系。结论流感病毒通过其NS1蛋白上调COX-2的表达。
Objective To explore the effect of Influenza virus on the expression of cyclooxygenase-2（COX-2） and its regulatory mechanism.Methods A549 cells were infected with influenza virus and co-transfected with plasmid carrying NS1 gene and reporter plasmid p COX-2-Luc containing the luciferase gene of COX-2 promoter,luciferase activity was measured,expression of COX-2 was measured by real-time polymerase chain reaction（RT-PCR） and western blot.Results Influenza virus upregulates the expression of COX-2 at mRNA and protein levels,NS1 protein enhances COX-2 gene promoter activity,COX-2 mRNA and protein expression in a concentration-dependent nanner.Conclusion Influenza virus upregulates the expression of COX-2 via with its NS1 protein.
出处
《中国卫生检验杂志》
北大核心
2014年第22期3283-3285,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省省市共建医药卫生专项基金计划(2010SSA003)
关键词
流感病毒
非结构蛋白NS1
环氧合酶2
Influenza virus
Non structural protein（NS1 protein）
Cyclooxygenase-2（COX-2）
