摘要
为了快速定量检测血液中降钙素原（Procalcitonin,PCT）含量,首先制备CdSe/ZnS量子点,再将量子点标记抗抗钙素单抗,标记单抗后量子点荧光发射峰从620nm红移至625nm,峰形保持良好。以制作的量子点标记免疫层析试纸条及其相应荧光测定系统。因减少单抗用量可降低试纸条成本,本研究采用结合垫上喷涂适量量子点,标记单抗并仅有检测线的结构。通过检测信噪比,优化此试纸条的参数。本研究的量子点荧光标记试纸条及其配套测定系统可在20min内快速检测PCT,定量检测线性范围0.2-100μg/L,灵敏度达到0.1μg/L。22份血液样本检测结果与目前商品设备检测结果的Pearson相关系数为0.9995,K-S检验的Sig为1.0,说明两种检测方法的一致性。快速定量检测PCT具有定量测量细菌性感染、临床指导抗生素合理应用的重要意义。
CdSe/ZnS quantum dots（QDs）were prepared and covalently linked to anti-katacalcin monoclonal antibodies. After modification, the QDs＆#39; maximum emission wavelength was shifted to 625 nm from 620 nm while maintaining the spectral properties. Then the QDs labeled lateral flow strip and corresponding fluorescence measuring instrument were designed and fabricated. To reduce the cost of strip by reducing the amounts of monoclonal antibodies, appropriate amounts of QDs labeled monoclonal antibodies were sprayed on the conjugation pad, with just one test line on the strip but without the control line. Parameters of the strip were optimized by measuring the signal to noise ratio. By using the strip and fluorescence measuring instrument, procalcitonin （PCT） could be detected in 20 min, and the quantitative detection range was 0. 2-100 μg/L with sensitivity of 0. 1 μg/L. A total of 22 blood samples were measured by both our method and the commercial instrument used in the hoptital. The results were consistent for their Pearson correlation coefficient （0. 9995） and Kolmogorov-Smirnov test （Sig=1. 0）. The rapid quantitative detection method for PCT is of great importance to quantitative detection of bacterial infection and rational usage of antibiotics clinically.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2014年第11期1592-1597,共6页
Chinese Journal of Analytical Chemistry
基金
深圳市科技研发基金(No.JCYJ20120828091734059)
国家自然科学基金(No.11404222)资助
关键词
量子点
免疫层析
降钙素原
血液
试纸条
Quantum dot
Immunochromatography
Procalcitonin
Blood
Lateral flow strip
