摘要
通过制备链霉亲和素修饰的CdTe量子点探针,建立了基于量子点探针的银增强显色可视化检测方法,并结合蛋白质芯片分析技术,将此方法应用于反相蛋白芯片的分析检测。以人IgG测定为模式,在醛基片上固定人IgG,加入10ngBio-羊抗人IgG及CdTe-SA探针,银增强显色15min。结果显示,人IgG样品量在100pg～1.6ng范围内,其对数值与信号灰度值呈良好的线性关系(r=0.997),检出限为50pg。本方法可实现微量蛋白的灵敏检测,具有操作简单,对仪器要求低,结果可视化等特点。
CdTe quantum dot probe modified with streptavidin （SA） was synthesized and prepared.A novel visual detection method based on the quantum dot coupled with silver enhancement was successfully established.Combined with the protein microarray analysis technique,this novel method did well in the detection of the reverse phase protein microarray.To detect the analytical performance,human IgG was used as a mode in this experiment.First,human IgG was immobilized on Aldehyde slides,then the optimized bio-goat anti-human IgG and CdTe-SA probe was added,finally silver enhancement coloration was carried out for 15 min.The results showed a good linear correlation between the signal gray level and the logarithm of human IgG immobilized on slides from 100 pg to 1.6 ng with a correlation coefficient （R2） of 0.997 while its lowest detectable limit was about 50 pg human IgG.This novel method could be used to the sensitive detection of micro-amount protein and showed the characteristics of the simple operation,lower request to the instruments and the visual results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第10期1383-1387,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.20975050
20575079)
973项目子课题(No.2006CB910803)
蛋白质组学国家重点实验室开放课题
江苏省"六大人才高峰"项目资助
中国药科大学研究生专项经费(No.JKY20009063)项目资助
关键词
量子点探针
银增强显色
可视化
蛋白芯片
Quantum dot probe
Silver enhancement
Visual detection
Protein microarray
