摘要
以巨玫瑰葡萄花粉为试材,通过TTC法研究了不同发育阶段、干燥方式、贮藏条件、贮藏时间4个因素对巨玫瑰葡萄花粉生活力的影响,并采用培养基萌发法观察花粉萌发过程。结果表明:巨玫瑰葡萄盛花期花粉生活力(79.99%)明显高于初花期花粉生活力(62.59%)和末花期生活力(29.57%);室内自然阴干的花粉生活力(60.92%)平均高于干燥器干燥的花粉生活力(53.85%);常温、0℃、4℃及-18℃条件下贮藏的花粉平均生活力随温度降低呈升高趋势;花粉生活力随贮藏时间的增长逐渐减弱,-18℃贮藏30 d的花粉生活力可达26.55%。因此,盛花期花粉自然阴干是花粉的最佳采集方式,而长时间贮藏则以低温保存为宜。
The effects of development stage,desiccating methods,storage conditions and storage time on Jumeigui grape pollen viability were studied by TTC,and pollen germination process was observed in medium.The results showed that：grape pollen viability in flowering season （79.99 ％） was higher than that at beginning of blooming period （62.59 ％） and end of flowering （29.57 ％）.Average pollen viability by the shade drying （60.92 ％） was higher than that by in desiocator drying （53.85 ％）.Average pollen viability incresed with temperature decreased in the condition of room temperature,0 ℃,4 and-18 ℃.The pollen viability stored in the condition in-18 ℃ for 30 days was 26.55 ％.Therefore,the pollen in florescence should be placed in the shade to dry and the low temperature was the optimal storage conditions for long time.
出处
《西南农业学报》
CSCD
北大核心
2014年第5期2115-2118,共4页
Southwest China Journal of Agricultural Sciences
基金
四川省"十二.五"育种攻关专项资金项目(2011NZ0098-8)
四川省财政基因工程专项资金项目(2011YZGG05-016-7)
关键词
巨玫瑰葡萄
花粉
贮藏条件
生活力
Jumeigui grape
Pollen
Desiccating method
Viability
