摘要
目的：探讨炮制、粉碎、分煎与合煎对莱菔子、苦杏仁及其药对中水、醇溶性总成分，芥子碱，脂肪油溶出的影响。方法：采用HPLC测定芥子碱溶出量，流动相乙腈-0．1％磷酸水溶液（15：85），检测波长326nm；重量法测定水、醇溶性浸出物及脂肪油溶出量。利用GC—MS技术鉴定苦杏仁脂肪酸组成及含量，DB-5石英毛细管色谱柱（0．25mm×30m），进样口温度230℃，气化室温度270℃，分流比50：1，程序升温（100～180℃，10℃·min^-1；180～200℃，4℃·min^-1；200～220℃，1℃·min^-1；220～270℃，5℃·min^-1），MS条件为EI离子源，70eV，扫描范围m／z30～500。结果：炮制有利于样品总成分及芥子碱的溶出，其中水溶性成分溶出最高增加9．61％，醇溶性成分141．03％，芥子碱20．00％；粉碎后样品中水、醇溶性成分与芥子碱的溶出量分别增加了0．36～1．85，38．41～62．06，1．40～2．60倍；分煎与合煎对样品中水、醇溶性成分溶出无明显影响，芥子碱溶出量合煎后降低45．16％～68．75％。结论：种子类中药如不粉碎不宜采用醇提取或入酒剂，粉碎能显著增加所测成分的溶出量，莱菔子和苦杏仁合煎不利于莱菔子中芥子碱的溶出。依法炮制与合理使用相结合才能确保中药疗效。
Objective： To explore effects of processing and usage on dissolution of water, alcohol soluble total components, sinapine and fatty oil from drug pairs of Raphani Semen and Armeniacae Semen Amarum. Method ： The content of sinapine was determined by HPLC, mobile phase was acetonitrile-0. 1% phosphoric acid solution （15： 85） and detection wavelength was set at 326 nm; contents of total components in water, alcoholsoluble extract and fatty oil were determined by weight method. Composition and content of fatty acids in Armeniacae Semen Amarum was analyzed by GC-MS with DB-5 quartz capillary column （0.25 mm × 30 m） , temperature of injection port and gasification room were 230℃ and 270 ℃ , respectively; split ratio was 50： 1, programmed temperature （100-180℃, 10 ℃·min^-1; 180-200 ℃, 4℃·min^-1; 200-220 ℃, 1℃·min^-1; 220-270℃ , 5℃·min^-1） , MS conditions for EI ion source, 70 eV and scanning range of m/z 30-500. Result： Total composition was easy to dissolving out by processing, dissolution of water, alcohol soluble ingredients and sinapine were 9.61% , 141.03% and 20.00% , while increase were 0.36-1.85, 38.41-62.06, 1.40-2.60 times after being crushed, respectively; dissolution between single-decoction and mixed-decoction had no significant differences, dissolution of sinapine reduced 45.16%-68.75% after mixed-decoction. Conclusion： If seeds Chinese medicine not shattered, it do not suitable for extracting by alcohol or wine, crushing can significantly increase solubility of these measured components, mixed-decoction of Raphani Semen and Armeniacae Semen Amarum againsts dissolution of sinapine. Processing in accordance and reasonable application must be combined to ensure curative effect of traditional Chinese medicine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第23期6-9,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173554)
关键词
莱菔子
苦杏仁
芥子碱
浸出物
脂肪油
Raphani Semen
Armeniacae Semen Amarum
sinapine
extractives
fatty oil
