摘要
目的了解北京地区婴幼儿病毒性腹泻的病原学特点。方法采集2011年1月-12月5岁以下腹泻患儿的粪便标本并填写个案调查表,用ELISA试剂盒检测A组轮状病毒,采用逆转录-聚合酶链反应(RT-PCR)检测人杯状病毒和星状病毒,采用PCR检测腺病毒。结果 604例粪便标本中,A组轮状病毒检出率15.89%,人杯状病毒检出率18.71%,星状病毒检出率2.98%,腺病毒检出率4.80%,病毒混合感染27例。11月份轮状病毒检出率最高,10月份人杯状病毒检出率最高。结论 A组轮状病毒和人杯状病毒为北京地区秋冬季婴幼儿腹泻的主要病原。
Objective To investigate the etiological features of viral diarrhea among children under 5years old in Beijing. Methods The stool specimens and clinical data were collected from 604 children under 5years old with acute diarrhea throughout 2011.Group A rotavirus was detected by ELISA,human calicivirus and astrovirus were identified by reverse transcription polymerase chain reaction（RT-PCR）,and adenovirus was detected by polymerase chain reaction（PCR）. Results Among 604 specimens,the detection rates of group A rotavirus,human calicivirus,astrovirus,and adenovirus was 15.89%,18.71%,2.98%,and 4.80%,respectively,and the mixed infections were found in 27 cases.The highest positive rates of group A rotavirus,human calicivirus were found in November and October. ConclusionGroup A rotavirus and human calicivirus were the leading cause for children under 5years old with diarrhea in Beijing in autumn and winter.
出处
《中国病原生物学杂志》
CSCD
北大核心
2014年第10期919-921,956,共4页
Journal of Pathogen Biology
基金
北京市自然科学基金项目(No.7132045)
关键词
婴幼儿
腹泻
病毒
病原学
Children
Diarrhea
Virus
Etiology
