摘要
以高效液相色谱法测定神安颗粒中五味子醇甲含量,建立神安颗粒多指标质量控制体系中脂溶性成分的控制指标。采用Kromasil C18色谱柱,流动相为甲醇-水（60∶40）,检测波长为250 nm,流速为1.0 m L/min,柱温为室温,进样量为10μL。结果五味子醇甲进样量在0.15~6.0μg范围内与峰面积积分值线性关系良好（R2=1）,平均回收率为97.38%（RSD=2.05%）,测定神安颗粒中五味子醇甲含量约为1.30 mg/g。本方法简便、快捷、专属性强、重现性好,可用于神安颗粒天麻苷类成分的质量控制。
The objective of this study was to develop a high performance liquid chromatography （HPLC） method for the determination of schisandrin in Shenan granules and hence to establish a quality control system of Shenan granules using liposoluble constituent as the controlling index. The chromatographic separation was performed on a Kromasil C18 column, the mobile phases consisted of methanol-water （60： 40）, the detection wavelength was 250 nm, flow rate was 1.0 mL/min,column temperature was room temperature, and the injection volume was 10μL. The experimental results showed that good linear relationship for schisandrin was established between peak area and injection amount of schisan-drin within the range of 0.15 - 6, 0μg （R2 = 1 ）, the average recovery was 97.38% （ RSD = 2.05% ）. Finally, the concentration of schisandrin in Shenan granules was determined to be around 1.30 mg/g. The developed HPLC method was simple, fast, specific and reproducible. It can be used for the quality control of Shenan granules.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第11期1815-1818,共4页
Natural Product Research and Development
关键词
高效液相色谱法
神安颗粒
天麻素
多指标质量控制体系
high performance liquid ehromatography
Shenan granules
sehisandrin
multitude index quality control system
