摘要
绿咖啡豆中的绿原酸共有九种同分异构体,所有同分异构体的总和称为总绿原酸,研究的主要目的是通过优化大孔吸附树脂纯化工艺将绿咖啡豆提取物中的总绿原酸含量由25%提高至70%以上,通过实验筛选合适的大孔吸附树脂,并优化树脂纯化的工艺参数如:上柱液浓度、上柱流速、解析溶剂度数、解析溶剂用量、解析流速等工艺指标,建立优化的纯化工艺路线。结果表明:上柱总绿原酸浓度为8mg/m L,上柱流速为1.0BV/h,解析用乙醇浓度为70%(v/v),乙醇用量为2BV,解析流速为1.5BV/h,经纯化后的总绿原酸经HPLC检测,总绿原酸含量可达71.23%,回收率可达87.46%。
Chlorogenic acid in green coffee beans of has a total of nine isomers, all of the isomers together are called total chlorogenic acid. The main purpose of this study is through the optimization of purification process by macroporous adsorption resin, total chlorogenic acid content increased from 25% to 70% above. First, suitable macroporous adsorption resin was screened, then, the optimized purification process parameters such as concentration, flow velocity through the column, the column fluid solvent concentration and the amount were dertermined. Results showed that the total chlorogenic acid concentration on the column was 8 mg/mL, the velocity 1.0 BV/h, elude ethanol concentration 70% （v/v）, the amount 2 BV, ethanol analytic velocity of 1.5 BV/h. After purification, the total chlorogenic acid was detected by HPLC. The total chlorogenic acid content was up to 71.23% and the recovery rate was 87.46%.
出处
《中国食品添加剂》
CAS
北大核心
2014年第7期123-128,共6页
China Food Additives
关键词
绿咖啡豆
总绿原酸
大孔树脂
纯化
green coffee beans
total chlorogenic acid
macroporous resin
purification
