摘要
将多化学指标与药效学指标相结合,优选蒲薏颗粒的最佳提取工艺。分别采用水提、水提醇沉、醇提和醇水双提4种工艺制备蒲薏颗粒样品。采用高效液相色谱法测定咖啡酸、大黄素和2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量,用紫外分光光度法测定多糖,薄层色谱法测定油酸。并通过药效学试验比较4种工艺样品对艾氏腹水癌小鼠生存期的影响。结果显示醇水双提所制得的蒲薏样品指标成分含量和药理作用均优于其他提取工艺所得样品。
Multi-chemical indexes combined with pharmacodynamics index were used to optimize the extraction technology of Puyi granules.Four kinds of extraction technology were investigated,which were water extraction,water extraction with ethanol precipitation,alcohol extraction,and alcohol-water double extraction.The content of caffeic acid,emodin and 2,3,5,4＇-tetrahydroxy-stilbene-2-O-β-D-glucoside were determined by HPLC,while polysaccharide and oleic acid were measured by UV and TLC,respectively.The pharmacodynamic effects of samples obtained from the above four processes on the survival time of mice bearing Ehrlich ascites carcinoma were compared.The results showed that alcoholwater double extraction was superior to other extractions in indicative ingredients and pharmacological effects.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2014年第11期1032-1035,1065,共5页
Chinese Journal of Pharmaceuticals
基金
贵州省中药现代化科技产业研究开发专项(黔科合中药字[2011]5081号)
贵州省中药现代化科技产业研究开发专项(黔科合重G字[2013]4001)
关键词
蒲薏颗粒
提取
多指标评价
艾氏腹水癌
Puyi granule
extraction
multiple indicators evaluation
Ehrlich ascites carcinoma
