摘要
为了对牛巴贝斯虫lytB基因进行克隆与序列分析,试验以兰州株牛巴贝斯虫lyt B基因组为模板进行PCR扩增,将扩增得到的特异性产物克隆到p GEM-T Easy载体上,并对其进行PCR检测、酶切鉴定及序列测定分析。结果表明:试验克隆得到的864 bp基因片段与Gen Bank收录的牛巴贝斯虫lyt B核苷酸序列同源性为97.8%。说明试验成功克隆出牛巴贝斯虫lyt B基因,与Gen Bank收录的牛巴贝斯虫lyt B核苷酸序列具有高度的同源性。
To perform the cloning and sequence analysis of the lytB gene in Babesia boris, the genome of the Lanzhou strains of Babesia boris was used as a template for PCR amplification. The specific amplified product was cloned into the pGEM -T Easy vector, and then the recombinant plasmid was used for PCR detection, restriction enzyme digestion and sequence analysis. The results showed that a 864 bp gene fragment was obtained by cloning, which shared high nucleotide sequence homology （97.8%） with that of the lytB gene in Babesia boris included in Gen- Bank. The results indicate that the lytB gene is successfully cloned, and shares highly homology with the nucleotide sequence of the lytB gene in Babesia bovis included in GenBank.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2014年第11期131-133,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
国家现代农业肉牛牦牛产业技术体系建设专项(CARS-38)
中央级科研院所基本科研业务费项目(1610322011005)
