摘要
研究了25℃、30℃和35℃三个出芝温度条件下,利用人工气候箱袋式栽培灵芝,其基质不同时期-部位的物质含量(总糖、还原糖、水溶性蛋白和游离氨基酸)与木质素酶系酶(漆酶和锰过氧化物酶)、纤维素酶系酶(羧甲基纤维素酶和滤纸酶)和酸性蛋白酶活性变化规律。结果显示,在25℃时,灵芝能够正常出芝,温度高于30℃以后子实体分化受到抑制;且在25℃条件下,不同时期-部位基质中总糖和可溶性蛋白含量低于其它2个温度处理组,而还原糖和游离氨基酸含量高于其它2个温度处理组;同时,不同时期-部位基质中木质素酶系和纤维素酶系酶活力低于其它2个温度处理组,但酸性蛋白酶活力高于另外其它2个温度处理组。提示袋料灵芝栽培时出芝温度控制在25℃左右利于其子实体生长发育。
Under 25℃,30℃ and 35℃ conditions,the changes of substance content（ total sugar,reducing sugar,water soluble protein and free amino acid）,lignin enzymes（ laccase and manganese peroxidase）,cellulose enzymes（ carboxymethyl cellulase and filter paper enzyme）,acid protease activity of bag culture substrate were studied during Ganoderma lucidum reproductive stage in different periods of different parts. The results showed that G. lucidum fruiting body could form at 25℃,and the formation was inhibited above 30℃. During G. lucidum reproductive stage,in different periods of different parts,the substrate total sugar and water soluble protein was lower at 25℃ than it at 30℃ and 35℃,and the reducing sugar and free amino acid contents was higher at 25℃ than it at the other two temperatures. The lignin enzymes and cellulase enzymes activity in the culture substrate at 25℃ was lower than it at the other two temperatures,and acid protease activity at 25℃ was higher than it at the other two temperatures. It provided that it was helpful for bag cultured G. lucidum fruiting body formation at about 25℃.
出处
《中国食用菌》
2014年第6期49-55,共7页
Edible Fungi of China
基金
国家农业产业技术体系药用菌栽培岗位建设项目,项目编号:农科教发[2007]12号
菌类药材研究与开发四川省科技创新团队,项目编号:2011JTD0021
四川省十二五育种攻关项目“菌类药材优质种质资源的收集及新材料的选育”,项目编号:2011NZ0098-12-04
四川省公益性科研院所基本科研项目“袋料灵芝营养代谢-药效成分时空关联模式的研究”,项目编号:A-2014N-6
关键词
灵芝
袋料
温度
出芝
酶活
Ganoderma lucidum
Bag culture
Temperature
Reproductive stage
Enzymes activities
