摘要
的研究缺氧诱导因子1（HIF-1α）、血管内皮生长因子（VEGF）、环氧化酶-2（COX-2）在乳腺癌中的表达与发生、发展及侵袭转移的关系。方法应用免疫组织化学s-P法检测6l例乳腺癌前病变患者[25例导管非典型增生（ADH）、36例导管原位癌（DCIS）]和78例乳腺浸润性癌患者（IDC）组织中HIF-1α、VEGF和COX-2的表达情况，并与52例乳腺增生患者标本作为对照。采用SPSS17．0软件包，HIF-1α，VEGF和COX-2样本阳性率组问比较采用Ⅳ。检验。结果（1）HIF-1α、VEGF和COX-2在乳腺IDC中的阳性表达率分别为67．9％、64．1％和79．5％，明显高于在乳腺癌前病变（DCIS为44．4％、44．4％、55．6％；ADH为28．0％、32．0％、36．0％）和乳腺普通导管增生病变（7．7％，23．1％，15．4％）中的表达，差异有统计学意义（Ⅳ。=5．696，12．424，28．415；P〈0．05）；（2）乳腺癌前病变中DCIS与ADH比较，HIF-1α（z11．699，P〉0．05）、VEGF（z10．957，P〉0．05）、COX-2（X^2=2．263，P〉0．05），差异无统计学意义；（3）HIF-1α、VEGF和COX-2在乳腺IDC中高表达与患者年龄（X^2=3．410，0．509，0．103；P〉0．05）、肿瘤大小（X^2=2．010，0．143，0．304；P〉0．05）无关，而与组织学分级（X^2=16．268，15．695，11．272；P〈0．05））和淋巴转移（矿=6．321，13．020，19．123；P〈0．05）相关；（4）HIF-1α、VEGF和COX-2三者在乳腺癌中的表达呈正相关（r=0．263，Y。=5．412，P〈0．05；r=0．436，Y19．441，P〈0．05）。结论HIF-1α、VEGF和COX-2高表达是乳腺癌发生、侵袭转移行为的重要生物学标志，HIF-1α可能发生在癌变早期阶段，是乳腺癌的早起事件；VEGF和COX-2与乳腺癌进展期侵袭转移更相关；HIF-1α、VEGF和COX-2联合检测可作为判断乳腺癌预后的指标。
Objective To investigate the expression of HIF-1 α,VEGF and COX-2 in breast carcinoma and their associations with the occurrence,development,invasion and metastasis of breast carcinoma. Methods The expression of HIF-1 α,VEGF and COX-2 was detected by immunohistochemistry S-P method in sixty-one cases of precancerous lesions including twenty-five cases of atypical ductal hyperplasia (ADH), thirty-six cases of ductal carcinoma in situ (DCIS),and seventy-eight cases of invasive ductal carcinoma (IDC)of the breast.Twenty-six cases of hyperplasia breast tissues were selected as control group.The differences of positive rates of HIF-1 α,VEGF and COX-2 were compared with chi-square test.Results The positive rates of HIF-1 α,VEGF and COX-2 in IDC were 67.9%,64.1 % and 79.5% respectively;the positive rates in DCIS were 44.4%,44.4%,55.6% respectively,and the positive rates in ADH were 28.0%,32.0%,36.0% respectively,which were higher than those in hyperplasia breast tissues(7.7%, 23.1 %,1 5.4%,χ2 =5.696,1 2.424,28.41 5;P <0.05).However,there were no significant differences in the positive expression rates of HIF-1 α,VEGF and COX-2 between ADH and DCIS tissue (χ2 =1 .699, 0.957,2.263;P>0.05).There was a correlation between over-expression of HIF-1 α,VEGF and COX-2 with the histological grade and lymph node metastasis of IDC repectively,wherease without correlation with age and size of tumor (χ2 =3.41 0,0.509,0.1 03;P >0.05 ).The expression of COX-2 was positively correlated with HIF-1 αand VEGF(r =0.263,χ2 =5.41 2,P<0.05;r =0.436,χ2 =9.441 ,P <0.05). Conclusions The overexpression of HIF-1 α,VEGF and COX-2 might be important biological markers for the invasion,lymph node metastasis and distant metastasis of IDC.The combined detection of HIF-1 α,VEGF and COX-2 is a predictor for prognosis.
出处
《中华诊断学电子杂志》
2014年第4期275-278,共4页
Chinese Journal of Diagnostics(Electronic Edition)
基金
日照市应用技术研究与开发计划项目(2060402)
济宁医学院科研计划重点项目(JY2013KJ051)
