摘要
目的：建立川贝止咳合剂的质量标准控制方法。方法：采用薄层色谱（TLC）法对方中所含枳壳、桔梗进行定性鉴别；采用高效液相色谱法测定方中柚皮苷的含量。结果：薄层色谱法可鉴别出枳壳、桔梗，且阴性无干扰；柚皮苷在160—800ng范围内呈良好线性关系，r=0．9999，平均回收率为98．90％，RSD=1．32％。结论：本方法操作简便、准确可靠、重现性好，可作为川贝止咳合剂的质量控制方法。
Objective：To establish the quality standard of Chuanbeizhike mixture. Methods：Thin layer chromatography （TLC） was used to identify Fructus Aurantii, Radix Platycodonis in Chuanbeizhike Mixture. Naringin in Fructus Aurantii was determined by high performance liquid chromatography （HPLC）. Results：The TLC spots of Fructus Aurantii and Radix Platycodonis were clear with good resolution and free of interference of negative samples. The calibration curve of HPLC was linear in the range of 160 ~ 800ng （ r =0.999 9）. The average recovery rate was 98.90%, RSD = 1.32%. Conclusion：The method is rapid, curate, sensitive and reliable, and it can be used to control the quality of Cbuanbeizhike Mixture.
出处
《世界中医药》
CAS
2014年第10期1355-1357,共3页
World Chinese Medicine
