摘要
目的建立活血胶囊(黄芪、红花、桃仁、枳壳、川芎、当归、赤芍、酸枣仁等)中羟基红花黄色素A和柚皮苷测定方法。方法色谱柱Agilent HC-C18色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-0.2%甲酸水(羟基红花黄色素A20∶80,柚皮苷33∶67),检测波长羟基红花黄色素A 403 nm,柚皮苷283 nm,体积流量0.8 mL/min,柱温25℃。结果羟基红花黄色素A在1.25～50μg/mL(R=0.999 9),柚皮苷在8.75～350μg/mL(R=1)质量浓度范围内峰面积具有良好的线性关系,方法回收率(n=6)分别为98.7%和99.2%,羟基红花黄色素A和柚皮苷的最低检出限分别为2.5 ng和2.75 ng。结论本方法操作简便,结果准确,重现性良好,可为活血胶囊的质量控制提供一定依据。
AIM To establish a method of determining hydroxy safflower yellow A and naringin in Huoxue Capsule（Astragali Radix,Carthami Flos,Persicae Semen,Aurantii Fructus,Chuanxiong Rhizoma,Angelicae sinensis Radix,Paeoniae Radix rubra,Ziziphi spinosae semen,etc.）.METHODS The determination was carried out with Agilent HC-C18 column.The mobile phase consisted of methanol-0.2% acetic（20∶ 80） for hydroxy safflower yellow A and（33∶ 67） for naringin.The detection wavelengths were 403 nm for hydroxy safflower yellow A,and 283 nm for naringin.The flow rate was 0.8 mL/min and the column temperature was 25 ℃.RESULTS There was a good linear relationship between the absorption peak area and the concentration in the range of 1.25-50 μg/mL（R=0.999 9） for hydroxy safflower A and 8.75-350 μg/mL（R=1） for naringin.The average recoveeries（n=6） were 98.7% and 99.2%,and the limit of detections were 2.5 ng and 2.75 ng,respectively.CONCLUSION The method is simple,accurate,and reproducible.It provides bases for the quality control.
出处
《中成药》
CAS
CSCD
北大核心
2011年第10期1733-1736,共4页
Chinese Traditional Patent Medicine
基金
陕西省"13115"科技创新工程重大科技专项(2009ZDKG-85)
陕西省自然科学基金(2010JM4047)
