摘要
目的采用体外模型对比研究观察、评估人椎间盘源性下腰痛(DLBP)椎间盘细胞外基质对小鼠背根神经节神经轴突生长的促进作用和P物质(SP)的诱导作用。方法从DLBP患者的椎间盘组织(实验组)以及腰椎骨折患者腰椎间盘组织(对照组)中提取细胞基质,分别检测神经生长因子(NGF)的含量,并对小鼠背根神经节神经元(DRG)进行培养,通过形态学观察神经轴突生长和酶联免疫吸附法检测SP含量。结果实验组NGF浓度显著高于对照组(P<0.05);神经轴突平均长度实验组为134.17μm±2.21μm,明显高于对照组的121.28μm±4.10μm,差异有统计学意义(P<0.05)。实验组中SP物质比率明显高于对照组(P<0.01)。结论人退变椎间盘细胞外基质中高表达的NGF能促进感觉神经轴突的生长以及诱导和疼痛相关神经肽的释放。
Objective To evaluate the axonal growth and induction of a painful neuropeptide, substance P （ SP） , u-sing rat dorsal root ganglion （ DRG） neurons and degenerated human disc cells in vitro. Methods The human inter-vertebral discs were harvested from patients with discogenic low back pain and normal people. Extracted medium from human degenerative intervertebral discs was cultured with neurons of rat DRGs. The promotion of axonal growth and SP induction of DRG neurons were evaluated in extracted medium using immunocytochemistry. Results The content of nerve growth factor （ NGF） in the discogenic low back pain was significantly longer than that in the normal people （P〈0. 05）. The average length of growing axons in the discogenic low back pain was 134. 17μm ± 2. 21μm. It was significantly longer than that in the normal people, which was 121. 28μm ± 4. 10 μm（P〈0. 05）. The percentage of SP-immunoreactive cells with growing axons was significantly higher only in the discogenic low back pain group com-pared with the anti-nerve growth factorβtreatment and normal groups （P〈0. 01）. Conclusions Extracted medium from the degenerative intervertebral dics promotes axonal growth. Furthermore, NGF promotes axonal growth and in-duces SP. These in vitro results may suggest that NGF promotes the growth of sensory nerve fibers innervating the de-generated intervertebral disc and may induce SP related with pain transmission.
出处
《临床骨科杂志》
2014年第5期601-603,共3页
Journal of Clinical Orthopaedics
基金
甘肃省自然科学基金(编号:2010GS04988)
关键词
腰椎间盘退变
神经生长因子
P物质
感觉神经
体外培养
下腰痛
degeneration of the lumbar intervertebral disc
nerve growth factor
substance P
sensory nerve fibers
vitro model
low back pain
