摘要
为了解安徽省山羊蓝氏贾第鞭毛虫的流行及基因型,本研究对安徽省六安、阜阳、宿州、池州、芜湖、滁州和亳州的506份山羊粪样采用卢戈氏碘液染色镜检法进行鉴定,对鉴定出的蓝氏贾第鞭毛虫阳性粪样,进行基于磷酸丙糖异构酶(TPI)和谷氨酸脱氢酶(GDH)基因的巢式PCR扩增,并对阳性产物进行测序,序列用Clustal X 1.81和Mega 5.05软件进行比对分析。镜检结果显示,共检出32份蓝氏贾第鞭毛虫阳性粪样,总感染率为6.3%。巢式PCR扩增结果显示,32份阳性粪样中,TPI基因扩增阳性23份,GDH基因扩增阳性16份,产物大小分别为530 bp和450 bp。序列分析表明,分离的虫株均为反刍动物特有的聚集体E,但基因亚型存在地区性分布差异,未发现具有人兽共患潜力的A型或B型。
Five hundred and six fresh fecal samples were collected from Lu＇an, Fuyang, Suzhou, Chizhou, Wuhu, Chuzhou and Bozhou in Anhui Province, and detected firstly by direct smear microscopy. The microscopy-positive samples were amplified by nested PCR targeting the triosephosphate isomerase （TPI） and glutamate dehydrogenase（GDH） genes. The positive PCR products were sequenced in both directions. The sequences were analyzed by ClustalX 1.81 for sequence alignment and the neighbor-joining trees were constructed by Mega 5.05. Thirty-two out of 506 fecal specimens were diagnosed as Giardia-positive by microscopy with an infection rate of 6.3%. 23 and 16 of the samples were typed as assemblage E by the TPI （530bp） and GDH （450bp） genes, respectively. These findings indicated that there was a different distribution of subtypes of assemblage E in different areas. The zoonosis genotypes such as assemblage A or B was not found in the present study.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2014年第5期401-403,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
安徽省"肉羊标准化生产与环境控制研究"项目
安徽省教育厅重大项目(No.KJ2014ZD09)
安徽科技学院兽医学重点学科(No.AKXK20101-2)
安徽科技学院校级重点项目(No.ZRC2014406)~~
