摘要
该研究采用正相硅胶柱色谱,反相柱色谱（RP-18）,凝胶色谱（Sephadex LH-20）以及重结晶等多种分离纯化手段,从杜仲雄花中分离得到9个化合物,通过化合物的理化性质和光谱数据鉴定其结构分别为：3-oxo-12-en-ursane-28-O-α-Larabinofuranosyl（1→6）-β-D-glucopyranoside（1）,2α,3β-dihydroxyurs-12-en-28-oic acid（28→1）-β-D-glucopyranosyl ester（2）,熊果酸（3）,α-香树脂醇（4）,熊果醇（5）,3-O-乙酰基熊果酸乙酸酯（6）,3-O-乙酰基齐墩果酸（7）,白桦脂酸（8）,白桦脂醇（9）。其中化合物1为新化合物,化合物2,4~7为首次从该科植物中分离得到。采用MTT法对所分离的9个化合物进行抗肿瘤活性测试,结果表明仅有化合物3对K562和Hep G2细胞显示出一定的抗肿瘤活性,其余化合物对所选的2种细胞均无明显活性。
Nine triterpenes compounds were isolated from the male flowers of Eucommia ulmoides by recrystallization and chromatographic techniques over silica gel, Sephadex LH-20, and RP-18 gel. Their chemical structures were identified on the basis of spectral analysis and as 3-oxo-12-en-ursane-28-O-α-Larabinofuranosyl（1→6）-β-D-glucopyranoside（1）,2α,3β-dihydroxyurs-12-en-28-oic acid（28→1）-β-D-glucopyranosyl ester （ 2）, ursolic acid （ 3 ）, α-amyrin （ 4 ）, uvaol （ 5 ）, ursolic acid acetate （ 6 ）, 3-O-acetate oleanoic acid （7）, betulinic acid （8）, and betulinol （9）. Compound 1 was a new compound, and compounds 2, 4-7 were isolated from the Eucommiu genus for the first time. Cytotoxic activity was tested for all the compounds against K562 and HepG2 cells. The results showed that only compound 3, exhibited cytotoxic activity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第21期4225-4229,共5页
China Journal of Chinese Materia Medica
基金
国家公益性行业专项基金项目(201004029)
关键词
杜仲雄花
三萜
抗肿瘤活性
Eucommia ulmoides
triterpene
cytotoxic activity
