摘要
通过获得热胁迫下茄子热应激表达功能基因,可为创制茄子耐热性品种提供研究基础。笔者采用抑制性消减杂交的方法构建了茄子耐热品系896的热胁迫基因上调表达文库,并对文库的部分序列进行了实时荧光定量PCR表达验证分析。构建文库信息分析表明,茄子受热胁迫后大量因子参与到代谢过程的调控中,主要参与糖类、脂类、能量及氨基酸代谢途径,体内的激素调控表现为生长抑制调控。获得文库中编码小分子热激蛋白的YP16,编码碳酸酐酶的YP61以及编码钙依赖蛋白激酶的YP248在茄子叶片受热胁迫后均上调表达。
Analysis of genes related to heat stress of eggplant can provide basis for cultivating heat tolerant varieties of eggplant. A SSH-cDNA library of heat-shocked eggplant 896 was constructed by suppression subtractive hybridization method. Real-time quantitative PCR expression analysis further validated the expression modes of partial sequences in this library. Library analysis showed that a large number of unigenes participated in the regulation of eggplant metabolic processes, including carbohydrate, lipid, energy and amino acid metabolic pathways, hormonal regulation of the heat-shocked eggplant expressed as growth inhibition in vivo. YP16 putative encoding a small heat shock protein, YP61 putative encoding carbonic anhydrase and YP248 putative encoding calcium-dependent protein kinase were confirmed to have up-regulated expression in eggplant leaves after the heat-shock.
出处
《中国农学通报》
CSCD
2014年第28期105-110,共6页
Chinese Agricultural Science Bulletin
基金
重庆市自然科学基金计划重点项目"茄子热胁迫基因表达谱中的文库构建与功能基因的挖掘"(CSTC
2011BA1032)
现代农业产业技术体系建设专项资金"国家大宗蔬菜产业技术体系岗位专家"(CARS-25-A-06)
