摘要
目的建立高效液相色谱法(HPLC)同时测定小儿腹泻散醇提物中胡椒碱和吴茱萸次碱的方法。方法采用高效液相色谱法,色谱柱KromasilC18(4.6mm×250mm,5μm);流动相为乙腈-水(36:64);流速:1mL/min;检测波长:344nm;柱温:30℃。结果胡椒碱的平均回收率为102.1%,RSD=3.9%;吴茱萸次碱的平均回收率为96.2%,RSD=1.5%。结论该方法准确稳定,重现性好,操作简单。
Objective To establish a method for the determination of pipeline and rutaecarpine in the alcohol extract of Powder for trea- ting infantile diarrhea by HPLC; Methods Using high performance liquid chromatography （HPLC） method with chromatographic column Kromasil C18 （4.6mm × 250mm,5μm） ; Chromatographic pure acetonitrile with water as mobile phase. Detection wavelength was 344nm , flow rate was l mL/min and the column temperature was at 30℃. Results The average recovery of piperine was 102.1% , RSD was 3.9% ; The average recovery of rutaecarpine was 96.2% , RSD was 1.9%. Conclutions The method for determination of piperine and rutaecarpine was accurate,simple and stable with good reproducibility.
出处
《湖北中医药大学学报》
2014年第5期48-50,共3页
Journal of Hubei University of Chinese Medicine
